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HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection)

HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection) application for HPLC

材料

分析色谱柱

产品编号
说明
价格

Ascentis® Express HILIC, 2.7 μm HPLC色谱柱

2.7 μm particle size, L × I.D. 10 cm × 3 mm

标准

产品编号
说明
价格

咖啡因

Sigma Reference Standard, vial of 250 mg

山梨酸

99.0-101.0% anhydrous basis

盐酸吡哆素 (B6)

analytical standard

尼克酰胺(烟酰胺)

analytical standard

核黄素 (B2)

analytical standard

SS苯甲酸

2000 μg/mL in dichloromethane, analytical standard

CONDITIONS

column

Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)

flow rate

0.6 mL/min

pressure

812 psi (56 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

2 μL

sample

Dilute 1:9 in acetonitrile

说明

分析说明

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UVand ELSD detectors in series, this chromatogram being from the UV.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany