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  • IL13 activates autophagy to regulate secretion in airway epithelial cells.

IL13 activates autophagy to regulate secretion in airway epithelial cells.

Autophagy (2015-06-11)
John D Dickinson, Yael Alevy, Nicole P Malvin, Khushbu K Patel, Sean P Gunsten, Michael J Holtzman, Thaddeus S Stappenbeck, Steven L Brody
摘要

Cytokine modulation of autophagy is increasingly recognized in disease pathogenesis, and current concepts suggest that type 1 cytokines activate autophagy, whereas type 2 cytokines are inhibitory. However, this paradigm derives primarily from studies of immune cells and is poorly characterized in tissue cells, including sentinel epithelial cells that regulate the immune response. In particular, the type 2 cytokine IL13 (interleukin 13) drives the formation of airway goblet cells that secrete excess mucus as a characteristic feature of airway disease, but whether this process is influenced by autophagy was undefined. Here we use a mouse model of airway disease in which IL33 (interleukin 33) stimulation leads to IL13-dependent formation of airway goblet cells as tracked by levels of mucin MUC5AC (mucin 5AC, oligomeric mucus/gel forming), and we show that these cells manifest a block in mucus secretion in autophagy gene Atg16l1-deficient mice compared to wild-type control mice. Similarly, primary-culture human tracheal epithelial cells treated with IL13 to stimulate mucus formation also exhibit a block in MUC5AC secretion in cells depleted of autophagy gene ATG5 (autophagy-related 5) or ATG14 (autophagy-related 14) compared to nondepleted control cells. Our findings indicate that autophagy is essential for airway mucus secretion in a type 2, IL13-dependent immune disease process and thereby provide a novel therapeutic strategy for attenuating airway obstruction in hypersecretory inflammatory diseases such as asthma, chronic obstructive pulmonary disease, and cystic fibrosis lung disease. Taken together, these observations suggest that the regulation of autophagy by Th2 cytokines is cell-context dependent.

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Sigma-Aldrich
驴血清
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IGEPAL® CA-630, for molecular biology
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脱氧胆酸钠, ≥97% (titration)
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抗乙酰化微管蛋白抗体,小鼠单克隆 小鼠抗, clone 6-11B-1, purified from hybridoma cell culture
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抗-LC3B 兔抗, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
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抗肌动蛋白抗体,克隆C4, clone C4, Chemicon®, from mouse
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抗-ATG5(N-末端) 兔抗, affinity isolated antibody, PBS solution
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IL-13 from rat, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)