Serum proteins of rats exposed to organic solvents examined by horizontal two-dimensional electrophoresis with an immobilized pH gradient in the first dimension.

Serum proteins of rats, exposed to methoxyethylacetate and a combination of methoxyethylacetate and isobutylacetate, were analyzed by horizontal high resolution two-dimensional gel electrophoresis with immobilized pH gradients in the first dimension. A total of 410 polypeptides were detected with either increasing or decreasing spot intensities after rat exposure to the organic solvents.