1,3-Dichloro-2-propanol induces apoptosis via both calcium and ROS in mouse melanoma cells.

We demonstrated that the apoptotic cell death of mouse melanoma cells exposed to 1,3- dichloro-2-propanol (DCP) was Ca(2+) dependent. 1,3-DCP inhibited the growth of mouse melanoma cells in a concentration-dependent manner. Furthermore, a terminal nick-end labeling of fragmented DNA (TUNEL) assay demonstrated that 1,3-DCP-induced apoptosis. Moreover, reactive oxygen species and Ca(2+), generated by mouse melanoma cells that were exposed to 1,3-DCP, resulted in the activation of NF-jB and MAPKs. Finally, treatment with BAPTA, an intracellular Ca(2+) chelator, completely inhibited the apoptosis that was induced by 1,3-DCP treatment of B16F10 cells.