Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Journal of agricultural and food chemistry (2004-08-19)

Dawn M Chapman, John H Thorngate, Mark A Matthews, Jean-Xavier Guinard, Susan E Ebeler

摘要

A rapid and automated solid phase microextraction (SPME) stable isotope dilution gas chromatography/mass spectrometry (GC-MS) method for 2-methoxy-3-isobutylpyrazine (MIBP) quantification in red wine was developed. Wines with 30% (w/v) NaCl and 2-methoxy-(2)H(3)-3-isobutylpyrazine internal standard were sampled with a 2 cm divinylbenzene/carboxen/poly(dimethylsiloxane) SPME fiber for 30 min at 40 degrees C and analyzed by GC-MS. The method was used to measure MIBP concentrations in Cabernet Sauvignon wines that were produced from six winter pruning treatments over two vintages. MIBP concentrations were significantly negatively correlated with buds per vine. In addition, the MIBP concentration was directly related to sensory vegetal intensity ratings obtained by descriptive analysis.

材料

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品牌

产品描述

57348-U

Supelco

SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 24 ga, StableFlex

57328-U

Supelco

SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 24 ga, for use with manual holder

57298-U

Supelco

SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with autosampler

57299-U

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SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder or autosampler, fiber L 2 cm

57329-U

Supelco

SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler

20358

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ORBO^™ Carboxen-564 管, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

20355

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ORBO-78 400/200mg，每包 25 支, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

10478-U

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Carbopack^®吸附剂, matrix Carboxen^® 1000, 60-80 mesh, bottle of 10 g

25467

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Carboxen^®1010 多孔层壁涂开管柱, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57318

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SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), d_f 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga

57295-U

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SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler

25461

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Carboxen^® 1006 多孔层壁涂开管柱, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57319

Supelco

SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler

57335-U

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SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler

24246

Supelco

Carboxen^®1010 多孔层壁涂开管柱, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

W313203

Sigma-Aldrich

2-异丁基-3-甲氧基吡嗪, ≥99%, FG

57334-U

Supelco

SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 24 ga, for use with manual holder, StableFlex fiber

47528-U

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2-异丁基-3-甲氧基吡嗪溶液, certified reference material

57344-U

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SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with manual holder

57343-U

Supelco

SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with autosampler

297666

Sigma-Aldrich

2-异丁基-3-甲氧基吡嗪, 99%

11072-U

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Carbopack^®吸附剂, matrix Carboxen^® 572, 20-45 mesh, bottle of 10 g

24241-U

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28718-U

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10477-U

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10269

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10471

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Carbopack^®吸附剂, matrix Carboxen^® 1003, 40-60 mesh, bottle of 10 g

57914-U

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SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), for use with autosampler, needle size 23 ga, metal alloy fiber, fiber L 2 cm

54041-U

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Carboxen^® 1000 双向固相萃取管, 200 mg/mL, pk 30

23446-U

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ORBO^™-93吸附管, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea