跳转至内容
Merck
CN
所有图片(1)

文件

G006300

LC/MS Analysis of Aflatoxin M1 in Milk on Ascentis® Express HILIC after SPE using Supel™ Tox AflaZea, Quantifying Picogram Concentrations

application for SPE, application for HPLC

登录查看公司和协议定价
所有图片(1)

技术

HPLC: suitable
solid phase extraction (SPE): suitable

试验参数

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: cow milk
SPE tube/cartridge: Supel™ Tox AlflaZea, 6 mL (55314-U)
extraction process: centrifuge 45 g liquid milk for 60 min at 5000 rpm (RCF 4863 xg), cool at 0 °C for 30 minutes, remove fat layer, transfer middle layer to collection flask,
sample addition: 10 mL, 2 drop/second using vacuum
washing: 2 x 2 mL acetonitrile (collect with sample)
eluate post-treatment: evaporate to dryness at 70 °C using nitrogen
sample preparation: reconstitut in 500 μL 20:80 acetonitrile:water, filter into 750 μL poly vial using PVDF filter, 0.2 μm
column: Ascentis Express C18, 50 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase: [A] 0.1% (v/v) formic acid; [B] acetonitrile
gradient: 90% A for 0.5 min; to 10% A in 0.5 min; held at 10% for 3 min; to 90% A in 0.10 min; held at 90% A for 3 min
flow rate: 0.4 mL/min
column temp.: ambient
detector: MS/MS (MRM transitions)
injection: 10 μL

适用性

application for HPLC
application for SPE

应用

food and beverages

分析说明

Sample pretreatment: centrifuge 45 g liquid milk for 60 min at 5000 rpm (RCF 4863 xg), cool at 0 °C for 30 minutes, remove fat layer, transfer middle layer to collection flask, add 20 mL acetonitrile to 50 mL centrifuge tube, add 20 mL of sample to acetonitrile, add contents of 1 Supel Q non-buffered tube, immedately disperse salt, quicky place on shaker for 5 minutes, centrifuge at 5000 rpm for 20 min, remove top layer (loading solution)
Aflatoxin M1 is the metabolite of Aflatoxin B1 and can be found in milk products when animals have been fed alatoxin-contminated feeds. Aflatoxins are positively associated with hepataocellular carcinomas. Aflattoxin M1 was detected in milk and quantified at concentrations fo 25 pg/mL. Sample preparation included a protein precipitation with solvent partitionng and sample clean-up using Supel Tox AflaZea SPE tubes. Analysis was perfomed via LC-MS using an Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm HPLC column for the separation. Detection and quanitification of aflatoxin M1 at 25 pg/mL satisfies the current regulatory requirements for aflatoxin M1 for EU treaty participants as stated in EC 1881/2006.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC

分析物

说明
    Aflatoxin M1 solution, 0.5 μg/mL in acetonitrile, analytical standard

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门