跳转至内容
Merck
CN
所有图片(1)

文件

G005922

HPLC Analysis of Aflatoxins B1, B2, G1 and G2 in Corn on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea and Fluorescence Detection

application for HPLC

登录查看公司和协议定价
所有图片(1)

技术

HPLC: suitable

试验参数

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: 25 g corn meal in 100 mL acetonitrile:water (84:16)
SPE tube/cartridge: Supel™ Tox AflaZea, 6 mL (55314-U)
sample addition: 2 mL
elution: collect sample in 5 mL silanized glass vial
eluate post-treatment: transfer 200 μL sample, add 300 μL TFA derivatization solution (70:20:10 water:TFA:acetic acid), heat in microreactor 20 min at 65 °C, cool, add 580 μL deionized water, vortex
column: Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (53825-U)
mobile phase: [A] water; [B] acetonitrile; (80:20, A:B)
flow rate: 0.350 mL/min
pressure: 325 psi (22.4 bar)
column temp.: 35 °C
detector: fluorescence, ex 360 nm, em 440 nm
injection: 20 μL

适用性

application for HPLC

应用

food and beverages

分析说明

Aflatoxins are closely related compounds produced as secondary metablolites of fungi. They are found in grains and nuts like corn, wheat or peanuts. Shown here is a rapid, sensitive method to extract these compounds from corn using SPE cartridges specifically designed for aflatoxins, Supel Tox AflaZea (aflatoxin zearalenone), which removes interferences from grains, feeds, TMR samples, peanuts, peanut products and aqueous solutions. Analysis was via HPLC on Ascentis Express Fused-Core C18 colums.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC

分析物

说明
    Aflatoxin G1 solution, 3 μg/mL in benzene:acetonitrile (98:2), analytical standard
    Aflatoxin B1 solution, 20 μg/mL in methanol, analytical standard
    Aflatoxin G2 solution, 3 μg/mL in benzene:acetonitrile (98:2), analytical standard
    Aflatoxin B2 solution, 3 μg/mL in benzene:acetonitrile (98:2), analytical standard

分析色谱柱

产品编号
说明
价格

相关产品

产品编号
说明
价格

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门