技术
HPLC: suitable
试验参数
column: Astec CHIROBIOTIC® R, 10 cm x 4.6 mm I.D., 5μm particles (13022AST)
mobile phase: 5:95, (water:methanol) with 0.3% acetic acid and 0.1% ammonium hydroxide
flow rate: 1 mL/min
pressure: 1700 psi (117 bar)
column temp.: 25 °C
detector: MS, ESI(-) TOF, SIM, m/z 147.03, split 100:1
injection: 10 μL
sample: DL-a-Hydroxyglutaric acid, 1 mg/mL in 50:50, water:methanol
适用性
application for HPLC
应用
clinical
分析说明
Chiral 2-hydroxyglutarates (2-OHG) are important molecular signatures of both healthy and diseased biological cells, their specific biochemical pathways and inborn errors of metabolism. The chiral differentiation and quantification of D-2-OHG and L-2-OHG is key for characterizing neurometabolic disorders like the 2-hydroxyglutaric acidurias that cause neurological impairment early in life. In this application, an Astec® CHIROBIOTIC® R column run under polar ionic mobile phase conditions achieved a rapid separation of the D and L enantiomers of 2-OHG under conditions amenable for sensitive LC-MS/MS detection.
法律信息
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC
分析物
说明
DL-α-Hydroxyglutaric acid disodium salt, ≥95.0% (GC)
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