技术
HPLC: suitable
试验参数
column: Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)
mobile phase: methanol
flow rate: 1.0 mL/min
pressure: 1653 psi (114 bar)
column temp.: 30 °C
detector: UV, 280 nm
injection: 5 μL
sample: 0.4 mg/mL tocopherol acetate, alpha-tocopherol and K1, 0.04 mg/mL D2 and D3, all in ethanol
适用性
application for HPLC
应用
vitamins, nutraceuticals, and natural products
分析说明
Analysis was conducted using standard solution, detection via UV.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Ergocalciferol (D2), analytical standard
Cholecalciferol (D3), analytical standard
DL-alpha-Tocopherol acetate, analytical standard
(±)-α-Tocopherol, analytical standard
Phylloquinone (K1), analytical standard
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