技术
HPLC: suitable
试验参数
column: Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)
mobile phase: [A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)
flow rate: 0.6 mL/min
pressure: 812 psi (56 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 2 μL
sample: Dilute 1:9 in acetonitrile
适用性
application for HPLC
应用
food and beverages
分析说明
This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UVand ELSD detectors in series, this chromatogram being from the UV.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Caffeine
Benzoic acid
Sorbic acid
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