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Merck
CN
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文件

G005745

HPLC Analysis of Mannose and Maltose Sugars on Ascentis® Express HILIC

application for HPLC

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About This Item

技术

HPLC: suitable

试验参数

column: Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase: [A] water; [B] acetonitrile; (25:75, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: ELSD, 55 °C, 3.5 bar nitrogen
injection: 10 μL

适用性

application for HPLC

应用

food and beverages

分析说明

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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