HPLC Analysis of Phenolic Compounds on Ascentis^® Express 2.7 μm RP-Amide

技术

HPLC: suitable

试验参数

column: Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)
mobile phase: [A] 20 mM phosphoric acid, pH 2; [B] acetonitrile; (75:25, A:B)
flow rate: 1.5 mL/min
column temp.: 35 °C
detector: UV, 270 nm
injection: 25 μL
sample: 50 mg/L in mobile phase

适用性

application for HPLC

应用

vitamins, nutraceuticals, and natural products