技术
HPLC: suitable
试验参数
column: Ascentis Express C8, 5 cm x 3 mm I.D., 2.7 μm particles (53848-U)
mobile phase: [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
gradient: 15 to 100% B in 8.5 min
flow rate: 0.6 mL/min
column temp.: 35 °C
detector: UV, 214 nm
injection: 2 μL
sample: 100 mg/L in 75 μM EDTA, 1.5 mM ascorbate, 15% acetonitrile
适用性
application for HPLC
应用
vitamins, nutraceuticals, and natural products
分析说明
This application demonstrates the suitability of Ascentis Express C8 for the efficient separation of phenolics from rosemary.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Rosmarinic acid
Carnosol
Carnosic acid
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