技术
HPLC: suitable
试验参数
column: Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
mobile phase: [A] hexane; [B] ethyl acetate
gradient: 10 to 30% B in 10 min; held at 30% B for 2 min
flow rate: 1.0 mL/min
column temp.: 30 °C
detector: UV, 290 nm
injection: 10 μL
适用性
application for HPLC
应用
vitamins, nutraceuticals, and natural products
分析说明
Ascentis silica is used in the analysis of fat-soluble vitamins using a normal phase gradient. These include vitamin K 3 (menadione), vitamin E (alpha and gamma tocopherol), and vitamin D3 (cholecalciferol).
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
(±)-α-Tocopherol, analytical standard
Menadione (K3), analytical standard
(+)-γ-Tocopherol, analytical standard
Cholecalciferol (D3), analytical standard
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