技术
HPLC: suitable
试验参数
column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: [A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 280 nm
injection: 5 μL
适用性
application for HPLC
应用
pharmaceutical (small molecule)
分析说明
This application demonstrates the suitability of the Ascentis C18 for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis Express C18 are presented
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Aminopterin
Folic acid
Methotrexate
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