LC/MS Analysis of Anesthetics on Ascentis^® Express F5

技术

HPLC: suitable

试验参数

column: Ascentis Express F5, 10 cm x 4.6 mm I.D., 2.7 μm particles (53590-U)
mobile phase: [A] 10 mM ammonium acetate, pH 4.0 with acetic acid; [B] acetonitrile; (20:80, A:B)
flow rate: 1.0 mL/min
pressure: 2320 psi (160 bar)
column temp.: 35 °C
detector: MS, ESI(+), SIR, m/z 235, 236, 247
injection: 2 μL
sample: 10 μg/mL in methanol

适用性

application for HPLC

应用

pharmaceutical (small molecule)