HPLC Analysis of Vitamin D₂ and Vitamin D₃on Ascentis^® C18

Name: HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18
Brand: SUPELCO

application for HPLC

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技术

HPLC: suitable

试验参数

column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase: acetonitrile
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 290 nm
sample: 50 μg/mL each in acetonitrile

适用性

application for HPLC

应用

clinical
vitamins, nutraceuticals, and natural products

分析说明

This application demonstrates the suitability of the Ascentis C18 for the analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis C18 are presented.