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Merck
CN

G005604

HPLC Analysis of Herbal Supplement Containing Milk Thistle on Ascentis® Express 2.7 μm C18

application for HPLC

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技术

HPLC: suitable

试验参数

column: Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] water with 0.1% formic acid; [B] methanol
gradient: held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min
flow rate: 0.6 mL/min
pressure: 4105 psi (283 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 20 μL
sample: 20 mg/mL in water:ethanol (5:95); sonicate 15 minutes; filter 0.45 μm; dilute to water:ethanol (80:20)

适用性

application for HPLC

应用

vitamins, nutraceuticals, and natural products

分析说明

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle containing herbal supplement.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

说明

    Taxifolin
    Silychristin
    Apigenin 7-glucoside
    Silydianin
    Quercitrin
    Silibinin
    Silybin B
    Isosilybin A
    Isosilybin B
    查看所有结果 (9)

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