HPLC Analysis of Maté Leaves on Ascentis^® Express C18

技术

HPLC: suitable

试验参数

sample/matrix: UAE (Ultrasonic Extraction) maté extract (dried and minced leaves (7.5 g) from maté were extracted with 225 mL of methanol in an ultrasonic bath, thermostated at 75 ± 0.5 °C (potency: 90 W; frequency: 40 kHz))
column: Ascentis Express C18, 150 x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: [A] water, pH 3 with formic acid; [B] acetonitrile, pH 3 with formic acid
gradient: 0 to 5% B in 20 min; 5 to 40% B in 30 min
flow rate: 1 mL/min
column temp.: 25 °C
detector: PDA: range 215- 420 nm (8 μL detector cell volume, cell temp. 40 °C, sampling rate 12.5 Hz, time constant 0.025 s)
injection: 10 μL

适用性

application for HPLC

应用

vitamins, nutraceuticals, and natural products