HPLC Analysis of Furocoumarins on Ascentis^® Express RP-Amide

技术

HPLC: suitable

试验参数

column: Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)
mobile phase: [A] water; [B] methanol; (40:60, A:B)
flow rate: 1.0 mL/min
pressure: 2843 psi (196 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 5 μL
sample: 25 μg/mL in water:methanol (90:10)

适用性

application for HPLC

应用

vitamins, nutraceuticals, and natural products