GC Analysis of Resveratrol in Red Wine on SLB^®-5ms after SPME using 85 μm Polyacrylate Fiber

技术

gas chromatography (GC): suitable
solid phase microextraction (SPME): suitable

试验参数

sample/matrix: 3 mL of red wine (California merlot) diluted 3:1 in 12% ethanol:water
SPME fiber: 85 μm polyacrylate FS 23 gauge auto (57294-U)
SPME fiber: 85 μm polyacrylate FS 24gauge auto (57305)
SPME fiber: 85 μm polyacrylate FS 24gauge manual (57304)
extraction: immersion for 15 min at room temperature while stirring at 400 rpm, fiber gently blotted with a Kimwipe to remove excess water, (then derivatization by immersion in a 4 mL vial containing 5 μL of Sylon-BFT (fresh vial for each analysis than was allowed to equilabrate 60-90 minutes prior to use) for 20 min)
desorption process: 280 °C for 2 min
column: SLB-5ms; 30 m x 0.25 mm I.D., 0.25 μm (28471-U)
oven: 100 °C (1 min), 10 °C/min to 325 °C (3 min)
MSD interface: 325 °C
scan range: m/z 40-450
carrier gas: helium, 1 mL/min, constant
liner: 0.75 mm I.D., SPME

适用性

application for SPME

应用

food and beverages