技术
HPLC: suitable
solid phase extraction (SPE): suitable
试验参数
sample preparation: SPE (Solid Phase Extraction)
sample/matrix: human urine spiked with piroxicam (4 μg/mL) and 2-aminopyridine (10 μg/mL)
SPE tube/cartridge: Discovery DSC-MCAX, 100 mg/3 mL (52783-U)
condition: 1 mL methanol; 1 mL 10 mM potassium phosphate, pH 3
sample addition: 1 mL
washing: 1 mL 10 mM potassium phosphate, pH 3; 1 mL methanol
elution: 1 mL 5% ammonium hydroxide in methanol
eluate post-treatment: evaporate to dryness with nitrogen at room temperature, reconstitute in 1 mL mobile phase
column: Discovery HS F5, 15 cm x 4.6 mm I.D., 5 μm particles (567516-U)
mobile phase: [A] 10 mM potassium phosphate, pH 6: [B] acetonitrile (85:15, A:B)
flow rate: 2 mL/min
column temp.: 25 °C
detector: UV, 220 nm
injection: 10 μL
适用性
application for HPLC
application for SPE
应用
clinical
应用
Piroxicam and 2-aminopyridine (piroxicam′s polar metabolite) was spiked into human urine at the levels of 4 and 10 μg/mL, respectively. The urine sample was diluted 1:1 with 10 mM potassium phosphate, pH 3.
法律信息
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