HPLC Analysis of Acrylamide in Fried Potato Chips on Discovery^® HS F5 after SPE using Discovery^® DSC-MCAX and DSC-18

技术

HPLC: suitable
solid phase extraction (SPE): suitable

试验参数

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: Finely grind 2.0 g of potato chips, place in 20 mL vial and add 10 mL water. Mix using vortex until mixture forms a thick paste. (Place sample in centrifuge tubes and centrifuge at 16.1 rcf for 5 min. Extract aqueous portion of sample leaving oil layer and solids.)
SPE tube/cartridge: Discovery DSC-MCAX, 300 mg/3 mL (52784-U)
SPE tube/cartridge: Discovery DSC-18, 1g/6mL (52606-U)
condition: connect a Discovery DSC-MCAX SPE tube to the top of a Discovery DSC-18 SPE tube using an SPE tube adapter, pass 1 mL methanol, then 1 mL DI water through both SPE tubes until the tubes are dry
sample addition: 1 mL of aqueous extract
washing: 1 mL DI water
elution: removing upper MCAX SPE tube, pass 2 mL methanol through lower DSC-18 SPE tube
eluate post-treatment: evaporate to dryness with nitrogen at 30 °C, reconstitute in 0.5 mL DI water
column: Discovery HS F5, 15 cm x 4.6 mm I.D., 3 μm particles (567507-U)
mobile phase: 100% ultra-pure water
column temp.: 35 °C
detector: ESI+
injection: 5 μL

适用性

application for HPLC
application for SPE

应用

food and beverages