技术
gas chromatography (GC): suitable
solid phase microextraction (SPME): suitable
试验参数
sample/matrix: 300-450g intact fruit in a 3 liter flask at ambient temp, exposed to an air flow of 25-30 mL/min
SPME fiber: polydimethylsiloxane, 100 μm film (57310-U)
desorption process: 250°C for 90 sec
column: 5% phenyl polysiloxane, 25m × 0.25 mm I.D., 0.34 μm film (Supelco equivalent, SLB-5ms, 28473-U)
oven: 40 °C (1.5 min) to 250 °C at 50 °C/min, hold 1 min
inj. temp.: 250 °C
detector: mass spectrometer, m/z = 40-300 (40 spectra/sec)
carrier gas: helium, 1.5 mL/min (44 cm/min)
sample: Apples
适用性
application for SPME
应用
agriculture
food and beverages
分析说明
SPME can reduce sampling time and is complementary to rapid separation/detection techniques such as time compressed gas chromatography (TCGC) and time-of-flight mass spectrometry (TOF-MS), to permit analysis of dozens of compounds in a few minutes. Investigators have used SPME to monitor flavor volatiles in apple, strawberry, tomato, and many other fruits and vegetables. In this application, 65 μm PDMS/divinylbenzene (DVB) SPME fibers were used to capture volatiles emanating from Mutsu apples stored for 3-4 months at 0 °C.
分析物
说明
Hexyl hexanoate
Isoamyl acetate
Hexyl 2-methylbutanoate
2-Methyl-1-butanol
α-Farnesene
Hexyl acetate
Butyl acetate
SPME 光纤
标准
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