技术
HPLC: suitable
试验参数
column: Discovery BIO PolyMA-WAX, 5 cm × 4.6 mm I.D., 5 μm particles (59602-U)
mobile phase: [A] 20 mM Tris-HCl, pH 8.0; [B] 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0
gradient: 5 to 70% B in 30 min. (linear)
flow rate: 0.5 mL/min
column temp.: 25 °C
detector: UV, 210 nm
injection: 10 mg hGH
适用性
application for HPLC
应用
life science and biopharma
分析说明
This Figure shows the degradation products of human growth hormone (hGH) well-separated on a Discovery BIO PolyMA-WAX anion-exchange column. The difference between the resolved compounds is the conversion of protein amide(s) to carboxylate(s), demonstrating both the power of the ion-exchange technique, and the efficiency of the Discovery BIO PolyMA-WAX column.
法律信息
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Human Growth Hormone human, HGH, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门