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物料
PE frit
polypropylene
组成
bed wt., 50 mg
包装
pk of 108
技术
solid phase extraction (SPE): suitable
体积
1 mL
杂质
<5% Water content
基质
polyamide resin base material
基质活性基团
amide, poly- phase
粒径
50-160 μm
pH值范围
4.5-7.5(surface pH)
堆积密度
0.2‑0.3 g/mL
分离技术
reversed phase
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一般描述
Sample Matrix Compatibility: Aqueous or methanolic solutions
- Polyamide Resin: Particle Size: 50-160 μm, Surf pH: 4.5-7.5, Density: 0.2-0.3 cm3/g, Water Content: < 5 %
- Useful for extracting gylcans from aqueous solutions.
- Used to adsorb polar compounds (-OH groups, esp. phenolic compounds) from aqueous or methanolic solutions under the reversed-phase mechanism through strong hydrogen bonding between compound hydroxyl groups and amide groups of the resin
- Also may be used for the extraction of tannins, chlorophyll, humic acid, pharmacologically active terpenoids, flavanoids, gallic acid, catechol A, protocatechuic acid, phloroglucinol, aromatic carboxylic acids, and nitroaromatic compounds
- Irreversibly retains quinones.
法律信息
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
商品
This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
Reversed-phase interaction will retain most molecules with hydrophobic character; it is very useful for extracting analytes that are very diverse in structure within the same sample.
实验方案
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.
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