跳转至内容
Merck
CN
所有图片(3)

文件

55261-U

Supelco

HybridSPE®-Phospholipid solid phase extraction (SPE) Cartridge

Cartridge, bed wt. 30 mg, volume 1 mL, pk of 100

别名:

HybridSPE (phospholipid and protein removal) SPE cartridge tube, 1 mL

登录查看公司和协议定价
所有图片(3)

About This Item

UNSPSC代码:
41115712
NACRES:
NB.21

product name

HybridSPE ®-磷脂SPE小柱, Cartridge, bed wt. 30 mg, volume 1 mL, pk of 100

物料

PE frit (20 μm)
polypropylene hardware

质量水平

100

产品线

HybridSPE®

组成

bed wt., 30 mg

包装

pk of 100

技术

solid phase extraction (SPE): suitable

体积

1 mL

基质活性基团

zirconia-based phase

正在寻找类似产品? 访问 产品对比指南

相关类别

一般描述

HybridSPE-Phospholipid磷脂去除技术是一种简单且通用的样本制备平台，设计用于在 LC-MS 或 LC-MS/MS 分析之前，从生物血浆和血清中总体去除内源性蛋白和磷脂干扰。首先通过加入和混合酸化乙腈使生物血浆或血清发生蛋白沉淀。然后通过离心除去沉淀蛋白，并将所得上清液上样至 HybridSPE-Phospholipid 96 孔板或固相萃取柱上，其作为化学过滤器，专门靶向去除内源性样品磷脂。截留磷脂的原理是HybridSPE-Phospholipid固定相键合的专有氧化锆离子和所有磷脂都具有的磷酸根发生高度选择性的路易斯酸碱相互作用。得到的洗脱液可立即用于 LC-MS 或 LC-MS-MS 分析。

HybridSPE-Phospholipid 96 孔板和Ultra固相萃取柱有“孔内”/“柱内”沉淀法可供选择。即先将生物血浆/血清加入孔内或柱内，接着加入酸化乙腈（沉淀剂）。短暂混合/涡旋后，抽真空。96孔板和 Ultra固相萃取柱含有一系列低孔隙率疏水滤膜/筛板，这些填充柱床的滤膜/筛板组件作为深层过滤器，有助于在提取过程中同时去除磷脂和沉淀蛋白。标准 HybridSPE-Phospholipid磷脂固相萃取小柱需要采用“离柱”沉淀法。

应用


  • Less is more: a methodological assessment of extraction techniques for per- and polyfluoroalkyl substances (PFAS) analysis in mammalian tissues.: This study assesses various extraction methods, including HybridSPE®-Phospholipid, for analyzing PFAS in tissues, highlighting efficiency and reproducibility (Mertens et al., 2023).

  • Rapid analysis of 65 pharmaceuticals and 7 personal care products in plasma and whole-body tissue samples of fish using acidic extraction, zirconia-coated silica cleanup, and liquid chromatography-tandem mass spectrometry.: This paper describes a rapid analytical method incorporating HybridSPE®-Phospholipid for sample preparation, demonstrating its application in environmental toxicology (Tanoue et al., 2020).


特点和优势

  • 与离线蛋白沉淀联合使用。
  • 融合了蛋白质沉淀的简单性和固相萃取(SPE)定向除去磷脂的选择性
  • 通过完全除去磷脂和沉淀蛋白，减少离子抑制
  • 2-3 步通用流程
  • 方法开发最少，甚至不需要方法开发
  • 有 96 孔和 1 mL 萃取柱规格可供选择

法律信息

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

Choose from one of the most recent versions:

分析证书(COA)

Lot/Batch Number
18931401
18686101
18640301
18500901
18265401

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Junji Ohnishi et al.
Human genomics, 11(1), 21-21 (2017-09-04)
Growing evidence suggests that spiritual/religious involvement may have beneficial effects on both psychological and physical functions. However, the biological basis for this relationship remains unclear. This study explored the role of spiritual/religious involvement across a wide range of biological markers
Sara Anjos et al.
Journal of proteome research, 18(8), 3174-3183 (2019-07-11)
Obesity is a public health problem and a risk factor for pathologies such type 2 diabetes mellitus, cardiovascular diseases, and nonalcoholic fatty liver disease. Given these clinical implications, there is a growing interest to understand the pathophysiological mechanism of obesity.
Takahiko Mitsui et al.
International urology and nephrology, 52(2), 233-238 (2019-10-07)
The present study was conducted to identify metabolites using a metabolomics approach and investigate the relationship between these metabolites and urgency as a major symptom of overactive bladder (OAB). In 47 male participants without any apparent neurological disease, OAB was
Seiichiro Sakao et al.
Molecular medicine reports, 23(4) (2021-01-27)
It is generally considered that there is an increase in glycolysis in the hypertrophied right ventricle (RV) during pulmonary hypertension (PH), which leads to a decrease in glucose oxidation through the tricarboxylic acid (TCA) cycle. Although recent studies have demonstrated
Marie Bagge Jensen et al.
Food chemistry, 345, 128835-128835 (2020-12-16)
Growing evidence of vitamin K's importance in human health beyond blood coagulation and bone health necessitates its further research. A method involving extraction, lipase treatment, clean-up, and detection and quantification by LC-ESI-MS/MS of phylloquinone (PK), menaquinone-4 (MK-4), menaquinone-7 (MK-7) and
查看所有相关文献

商品

Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid

This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.

Ion-Suppression & Phospholipid Contamination

An article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.

HybridSPE-Phospholipid Technology

This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.

实验方案

Enrichment of Phospholipids in Biological Samples Using HybridSPE-PL

A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.

相关内容

Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

小分子HPLC分析_小分子鉴定_液相色谱-默克生命科学

小分子特指低分子量化合物（通常小于900道尔顿），分离小分子方法包括高效液相色谱(HPLC)、液相(LC)、气相(GC)、薄层色谱(TLC) 和毛细管电泳(CE)。此外，还可通过磁共振波谱法(NMR)或 质谱法(MS)等方法鉴定小分子。

Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

Chromatograms

application for HPLC

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门