推荐产品
产品名称
BIOshell Glycan HPLC Columns, L × I.D. 15 cm × 2.1 mm, 2.7 μm particle size
物料
stainless steel hardware (column)
质量水平
Agency
suitable for USP L86
描述
shell thickness (0.5 μm)
solid core (1.7 μm)
产品线
BIOshell
特点
endcapped: no
分析物化学类别
glycans
制造商/商品名称
BIOshell
包装
1 ea of
参数
1000 bar max. pressure (14500 psi)
1000 bar pressure (14500 psi)
60 °C max. temp.
技术
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
长度 × 内径
15 cm × 2.1 mm
表面积
135 m2/g
基质
spherical silica particle platform
superficially porous particle
基质活性基团
hydroxy, penta- phase
粒径
2.7 μm
孔径
90 Å
工作pH范围
2-9
分离技术
hydrophilic interaction (HILIC)
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一般描述
View the BIOshell Glycan HPLC Columns Flyer
应用
特点和优势
法律信息
历史批次信息供参考:
分析证书(COA)
商品
The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.
This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
Workflows for monoclonal antibody adalimumab characterization ensure drug safety and efficacy through critical quality attribute analysis.
实验方案
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.
相关内容
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.
生物分子是活细胞产生的大分子聚合物,作为活生物体的基本组成部分执行许多生命必需的生物过程。尽管现有很多分离和分析技术可供选择,最常用的还是高效液相色谱法(HPLC)。
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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