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Merck
CN

50994-U

Supelco

BIOshell Glycan (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

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UNSPSC代码:
41115700
NACRES:
SB.52

product name

BIOshell Glycan HPLC Columns, L × I.D. 15 cm × 2.1 mm, 2.7 μm particle size

物料

stainless steel hardware (column)

质量水平

Agency

suitable for USP L86

描述

shell thickness (0.5 μm)
solid core (1.7 μm)

产品线

BIOshell

特点

endcapped: no

分析物化学类别

glycans

制造商/商品名称

BIOshell

包装

1 ea of

参数

1000 bar max. pressure (14500 psi)
1000 bar pressure (14500 psi)
60 °C max. temp.

技术

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

长度 × 内径

15 cm × 2.1 mm

表面积

135 m2/g

基质

spherical silica particle platform
superficially porous particle

基质活性基团

hydroxy, penta- phase

粒径

2.7 μm

孔径

90 Å

operating pH range

2-9

分离技术

hydrophilic interaction (HILIC)

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相关类别

一般描述

BIOshell Glycan HPLC Columns are specifically engineered to deliver fast, high resolution, reproducible glycan identification using HILIC chromatography. The Fused-Core columns provide improved resolution when compared to conventional LC methods thus allowing more reliable quantitation and confident identification of glycans.

View the BIOshell Glycan HPLC Columns Flyer

应用

BIOshell Glycan HPLC Columns are high-performance liquid chromatography columns with Fused-Core particle design. Their column chemistry is suitable for hydrophilic interactive liquid chromatography (HILIC) analysis of biomolecules like oligosaccharides.

特点和优势

BIOshell Glycan HPLC Columns are compatible both with UHPLC and HPLC systems. They are specifically engineered for the fast and high-resolution seperation of biomolecules. These columns operate at high flow rates and have 40% higher efficiency than fully porous particles of the same size. They are stable at high temperatures, which increases the throughput and enhances the peak shape.

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商品

The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

Workflows for monoclonal antibody adalimumab characterization ensure drug safety and efficacy through critical quality attribute analysis.

实验方案

A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

相关内容

High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.

生物分子是活细胞产生的大分子聚合物,作为活生物体的基本组成部分执行许多生命必需的生物过程。尽管现有很多分离和分析技术可供选择,最常用的还是高效液相色谱法(HPLC)。

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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