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50859-U

Supelco

Ascentis® Express 90 Å F5 (2 μm) HPLC Columns

L × I.D. 5 cm × 2.1 mm UHPLC Column

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别名:
Core-shell (SPP) Fused Core PFP HPLC column
UNSPSC代码:
41115700
NACRES:
SB.52

product name

Ascentis® Express F5, 2 μm UHPLC 色谱柱, 2 μm particle size, L × I.D. 5 cm × 2.1 mm

物料

stainless steel column

质量水平

100

Agency

suitable for USP L43

产品线

Ascentis®

特点

endcapped

制造商/商品名称

Ascentis®

包装

1 ea of

参数

1000 bar max. pressure (14500 psi)
60 °C temp. range

技术

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

长度 × 内径

5 cm × 2.1 mm

表面积

120 m2/g

杂质

<5 ppm metals

基质

Fused-Core particle platform
superficially porous particle

基质活性基团

PFP (pentafluorophenyl) phase

粒径

2 μm

孔径

90 Å pore size

工作pH值

2-8

应用

food and beverages

分离技术

reversed phase

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相关类别

一般描述

Ascentis® Express 2.0 μm F5是基于Fused-Core®颗粒设计的快速UHPLC柱。这种颗粒设计在0.4 μm厚的多孔壳中的扩散路径较浅，且总体粒径小至2 μm，因此柱效很高。Ascentis® Express 2.0 μm F5的键合封端的二甲基五氟苯基丙基固定相由于存在负电性氟而可提供含缺电子苯环的稳定的反相填充。除了形成π-π相互作用和温和的空间相互作用外，F5相还通过极性相互作用保留化合物。F5相具有极性和非极性特性，可表现出双模式保留行为，有时会随流动相中乙腈含量的变化而产生“ U形”保留，在ACN浓度低和高的情况下保留都会增加（ 反相和HILIC保留模式）。Ascentis® Express 2.0 μm F5可作为C18选择性的补充，对碱性、酸性或中性化合物进行分析。

应用

使用Ascentis® Express F5（2 μm）对霉菌毒素进行UHPLC/MS分析

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法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

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Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore
Maria A Khalikova et al.
Talanta, 130, 433-441 (2014-08-28)
The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
Imran Ali et al.
Journal of separation science, 35(23), 3235-3249 (2012-11-28)
New generation columns, i.e. packed with superficially porous silica particles are available as trade names with following manufacturers: Halo, Ascentis Express, Proshell 120, Kinetex, Accucore, Sunshell, and Nucleoshell. These provide ultra-fast HPLC separations for a variety of compounds with moderate
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 120, 112-119 (2016-01-01)
A new high-performance liquid chromatography method using fused-core column for fast separation of resveratrol and polydatin has been developed and used for quality control of nutraceuticals with resveratrol and polydatin content. Retention characteristics (log k) were studied under different conditions
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相关内容

Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

小分子HPLC分析_小分子鉴定_液相色谱-默克生命科学

小分子特指低分子量化合物（通常小于900道尔顿），分离小分子方法包括高效液相色谱(HPLC)、液相(LC)、气相(GC)、薄层色谱(TLC) 和毛细管电泳(CE)。此外，还可通过磁共振波谱法(NMR)或 质谱法(MS)等方法鉴定小分子。

Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

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