Anti-HLA-DRB1 Antibody, clone 4D12 ZooMAb^® Rabbit Monoclonal

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 4D12 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Human leukocyte antigen DRB1 (HLA-DRB1). It targets an epitope within 15 amino acids from the N-terminal extracellular domain of the protein.

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal extracellular domain of human HLA-DRB1.

Quality Control Testing

Evaluated by Western Blotting in Ramos cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected HLA-DRB1 in Ramos cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected HLA-DRB1 in lysates from human PBMCs and human tonsil tissue.

Affinity Binding Assay:: The binding affinity of this antibody to HLA-DRB1 peptide was at least one thousand-fold (1,000X) higher than that obtained with the non-specific control peptide.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HLA-DRB1 in Raji and Ramos cells.

Flow Cytometry Analysis: 1 µg from a representative lot detected HLA-DRB1 in human PBMC.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected HLA-DRB1 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

HLA class II histocompatibility antigen, DRB1 beta chain (UniProt: P01911; also known as Human leukocyte antigen DRB1) is encoded by the HLA-DRB1 gene (Gene ID: 3123) in human. HLA-DRB1 is a protein involved in the immune system′s recognition and presentation of foreign antigens to T cells. It belongs to the major histocompatibility complex (MHC) class II proteins. It consists of an alpha chain and a beta chain. The beta chain, encoded by the HLA-DRB1 gene, contains extracellular domains responsible for antigen binding and presentation to T cells. The primary function of HLA-DRB1 is to present antigenic peptides to CD4+ T cells, facilitating immune responses against pathogens. It plays a crucial role in adaptive immunity by presenting antigens derived from external sources, such as viruses or bacteria, to T lymphocytes. HLA-DRB1 is expressed on antigen-presenting cells (APCs) like dendritic cells, macrophages, and B cells. Its expression is regulated by various factors, including cytokines and immune stimulation. In different tissues, HLA-DRB1 expression can vary, indicating tissue-specific roles in immune responses. It forms complexes with other MHC class II molecules, chaperones, and accessory proteins in the endosomal-lysosomal pathway to facilitate antigen processing and presentation. HLA-DRB1 is associated with several autoimmune diseases, including rheumatoid arthritis and certain forms of juvenile idiopathic arthritis. Specific HLA-DRB1 alleles confer susceptibility or protection to these diseases and contribute to the pathogenesis by influencing antigen presentation and immune responses. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.