生物来源
rabbit
质量水平
重组
expressed in HEK 293 cells
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
2N6, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
产品线
ZooMAb® learn more
形式
lyophilized
分子量
calculated mol wt 24.77 kDa
observed mol wt ~28 kDa
纯化方式
using Protein A
种属反应性
human
种属反应性(根据同源性预测)
rat, mouse
包装
antibody small pack of 25
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
技术
affinity binding assay: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG
表位序列
C-terminal
Protein ID登记号
UniProt登记号
运输
ambient
储存温度
2-8°C
基因信息
human ... SOCS3(9021)
一般描述
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特异性
Clone 2N6 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Suppressor of cytokine signaling 3 (SOCS-3). It targets an epitope within 16 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human Suppressor of cytokine signaling 3 (SOCS-3).
应用
Quality Control Testing
Evaluated by Western Blotting in A375 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SOCS-3 in A375 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SOCS-3 in lysate from Human liver tissue and His/ABP-tagged recombinant fragment of Human SOCS-3.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SOCS-3 in human liver tissue sections.
Affinity Binding Assay Analysis: A representative lot of this antibody bound SOCS-3 with a KD of 9.2 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in A375 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SOCS-3 in A375 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SOCS-3 in lysate from Human liver tissue and His/ABP-tagged recombinant fragment of Human SOCS-3.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SOCS-3 in human liver tissue sections.
Affinity Binding Assay Analysis: A representative lot of this antibody bound SOCS-3 with a KD of 9.2 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
目标描述
Suppressor of cytokine signaling 3 (UniProt: O14543; also known as SOCS-3, Cytokine-inducible SH2 protein 3, CIS-3, STAT-induced STAT inhibitor 3, SSI-3) is encoded by the SOSC3 (also known as CIS3, SSI3) gene (Gene ID: 9021) in human. SOCS family of proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS-3 is widely expressed with high expression observed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. It is reported to serve as a tumor suppressor in breast cancer cells that is regulated by prolactin. It is involved in negative regulation of cytokines that signal through the JAK/STAT pathway leading to inhibition of STAT3 activation. It is reported to interact with multiple activated proteins of the tyrosine kinase signaling pathway. Its binding to JAK2 is mediated through the KIR (aa 22-33) and SH2 domains (aa 46-142) to a phosphorylated tyrosine residue within the JAK2 JH1 domain. SOCS-3 also contains a SOCS box domain (aa 177-224) that mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. Inflammation and infection are reported to stimulate SOCS-3 expression in different myeloid and lymphoid cell populations as well as in diverse non-hematopoietic cells. Genetic deletion of SOCS-3 leads to mid-gestational embryonic lethality due to increased STAT3 and MAP kinase activation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Carow, B., and Rottenberg, ME. (2014). Front. Immunol. 5: 58; Sasaki, A., et al. (1999). Genes Cell. 4(6); 339-351).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
重悬
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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