一般描述
QIAcard FTA micro format with one spot area to collect, stabilize, process, transport, and archive colored samples such as blood. FTA technology enables cell lysis on contact, denatures proteins and immediate stabilization and protection of nucleic acids.
FTA micro cards utilize QIAcard FTA technology that simplifies the handling and processing of nucleic acids. FTA Cards contain chemicals that lyse cells, denature proteins and protect nucleic acids from nucleases, oxidation and UV damage. FTA cards rapidly inactivate organisms, including blood-borne pathogens, and prevent the growth of bacteria and other microorganisms.
Convenient for protocols that require different locations for testing and archiving samples. Different samples can be processed independently.
特点和优势
- Includes a pink dye that turns white when a colorless sample is applied.
- One sample area for application of up to 125 μl sample volume per card.
- Simply apply your sample to the FTA Card. Cell membranes and organelles are lysed and the released nucleic acids are entrapped in the fibers of the matrix. The nucleic acids remain immobilized and are preserved for transport, immediate processing or long-term room temperature storage.
- Since captured nucleic acids are preserved, FTA Cards facilitate sample collection in remote locations and simplify sample transport.
- Ship your samples back to the laboratory without expensive special handling or dry ice and process at your convenience.
- FTA Cards can be used with virtually any sample type: Blood, Cultured cells, Buccal cells, Plasmids, and Solid tissue.
- Captured nucleic acids are ready for purification when you are. Just take a punch from the FTA Card, wash with FTA Purification Reagent and rinse with TE-1 (10 mM Tris-HCI, 0.1 mM EDTA, pH 8) buffer. DNA on the washed punch is ready to use in applications such as PCR, SNP analysis, and real-time PCR. Since PCR products remain in solution, the punch can be used for multiple amplifications.
其他说明
Field of Use : For internal research use only
法律信息
法规信息
商品
Description of common problems that can occur during genomic DNA preparation
This article describes the evaluation of Whatman FTA cards from Cytiva for their ability to collect, store, and isolate high-quality RNA from a variety of crude biological samples.
Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.
实验方案
This page shows genomic DNA purification from sample on FTA elute from Cytiva.
This document shows intructions for EasiCollect™ device from Cytiva.
This study examined the yield and quality of DNA from samples applied to Whatman FTA cards, using five common methods of DNA extraction.
A number of options are available for sample collection, transport, archiving, and DNA purification.
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