W0393
抗-WRN抗体，小鼠单克隆 小鼠抗
clone 195C, purified from hybridoma cell culture
别名:
抗-RECQ3, 抗-RECQL2, 抗-RECQL3, 抗-Werner综合征蛋白, 抗-Werner综合征解旋酶
产品名称
抗-WRN抗体，小鼠单克隆 小鼠抗, clone 195C, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
195C, monoclonal
form
buffered aqueous solution
mol wt
antigen ~167 kDa
species reactivity
human
concentration
~1 mg/mL
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 2-4 μg/mL using nuclear cell extract of HeLa cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... WRN(7486)
Application
WRN抗体还可用于
- 酶联免疫吸附测定（ELISA）
- 免疫印迹
- 免疫沉淀
- 免疫组化
- 蛋白印迹
Biochem/physiol Actions
Werner综合征ATP依赖性解旋酶（WRN）蛋白已被证明具有三种已知的催化活性：3′-5′ 解旋酶、核酸外切酶和ATP酶活性。该蛋白的C端区域包含核定位信号（NLS），因此将WRN蛋白靶向细胞核，核仁和核质灶。WRN参与多种DNA代谢过程表明其具有抑癌作用。WRN蛋白的突变会导致Werner综合征（WS）。WRN的表观遗传失活导致WRN-核酸外切酶活性丧失，导致染色体不稳定性增加和对化疗药物的超敏反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
抗WRN小鼠单克隆抗体（小鼠IgG1亚型）是由小鼠骨髓瘤细胞（p3-NS1/Ag4-1）和用制备自人WRN重组融合蛋白片段进行免疫的BALB/c小鼠脾细胞融合产生的杂交瘤195C生成的。Werner综合征ATP依赖性解旋酶（WRN）位于8号染色体上，为167 kDa。WRN蛋白属于DNA解旋酶的RecQ解旋酶家族。
Immunogen
人WRN的重组融合蛋白片段（氨基酸1074-1432）。
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Linda J W Bosch et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 22(18), 4612-4622 (2016-04-29)
WRN promoter CpG island hypermethylation in colorectal cancer has been reported to increase sensitivity to irinotecan-based therapies. We aimed to characterize methylation of the WRN promoter, determine the effect of WRN promoter hypermethylation upon expression, and validate a previous report
S Deepak Amalnath et al.
Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology, 36(4), 323-325 (2017-08-11)
Werner syndrome is a rare progeroid syndrome caused by the WRN gene mutation. It is characterized by a general appearance of premature aging, diabetes mellitus, and atherosclerosis, and an increased risk of malignancies. We report a patient who presented with
Werner complex deficiency in cells disrupts the nuclear pore complex and the distribution of lamin B1
Li Zhi, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1833(12), 3338-3345 (2013)
Piri Welcsh et al.
Methods (San Diego, Calif.), 108, 99-110 (2016-05-01)
Functional studies of the roles that DNA helicases play in human cells have benefited immensely from DNA fiber (or single molecule) technologies, which enable us to discern minute differences in behaviors of individual replication forks in genomic DNA in vivo.
Enzymatic and DNA binding properties of purified WRN protein: high affinity binding to single-stranded DNA but not to DNA damage induced by 4NQO
Orren D K, et al.
Nucleic Acids Research, 27(17), 3557-3566 (1999)
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