W0393
抗-WRN抗体，小鼠单克隆 小鼠抗
clone 195C, purified from hybridoma cell culture
别名:
抗-RECQ3, 抗-RECQL2, 抗-RECQL3, 抗-Werner综合征蛋白, 抗-Werner综合征解旋酶
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关于此项目
Conjugate:
unconjugated
Clone:
195C, monoclonal
Application:
ELISA (i), IHC, IP, WB
Citations:
13
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
195C, monoclonal
form
buffered aqueous solution
mol wt
antigen ~167 kDa
species reactivity
human
concentration
~1 mg/mL
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 2-4 μg/mL using nuclear cell extract of HeLa cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... WRN(7486)
General description
抗WRN小鼠单克隆抗体（小鼠IgG1亚型）是由小鼠骨髓瘤细胞（p3-NS1/Ag4-1）和用制备自人WRN重组融合蛋白片段进行免疫的BALB/c小鼠脾细胞融合产生的杂交瘤195C生成的。Werner综合征ATP依赖性解旋酶（WRN）位于8号染色体上，为167 kDa。WRN蛋白属于DNA解旋酶的RecQ解旋酶家族。
Immunogen
人WRN的重组融合蛋白片段（氨基酸1074-1432）。
Application
WRN抗体还可用于
- 酶联免疫吸附测定（ELISA）
- 免疫印迹
- 免疫沉淀
- 免疫组化
- 蛋白印迹
Biochem/physiol Actions
Werner综合征ATP依赖性解旋酶（WRN）蛋白已被证明具有三种已知的催化活性：3′-5′ 解旋酶、核酸外切酶和ATP酶活性。该蛋白的C端区域包含核定位信号（NLS），因此将WRN蛋白靶向细胞核，核仁和核质灶。WRN参与多种DNA代谢过程表明其具有抑癌作用。WRN蛋白的突变会导致Werner综合征（WS）。WRN的表观遗传失活导致WRN-核酸外切酶活性丧失，导致染色体不稳定性增加和对化疗药物的超敏反应。
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
低风险生物材料
此项目有
Enzymatic and DNA binding properties of purified WRN protein: high affinity binding to single-stranded DNA but not to DNA damage induced by 4NQO
Orren D K, et al.
Nucleic Acids Research, 27(17), 3557-3566 (1999)
Bidisha Saha et al.
Molecular genetics & genomic medicine, 1(1), 7-14 (2013-08-13)
Werner syndrome is a rare autosomal recessive disorder characterized by multiple features consistent with accelerated aging. It is caused by mutations in the WRN gene, which encodes a RecQ type helicase. To date, more than 70 disease-causing mutations have been
Class I histone deacetylase HDAC1 and WRN RECQ helicase contribute additively to protect replication forks upon hydroxyurea-induced arrest
Kehrli K, et al.
The Journal of Biological Chemistry, 291(47), 24487-24503 (2016)
Linda J W Bosch et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 22(18), 4612-4622 (2016-04-29)
WRN promoter CpG island hypermethylation in colorectal cancer has been reported to increase sensitivity to irinotecan-based therapies. We aimed to characterize methylation of the WRN promoter, determine the effect of WRN promoter hypermethylation upon expression, and validate a previous report
S Deepak Amalnath et al.
Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology, 36(4), 323-325 (2017-08-11)
Werner syndrome is a rare progeroid syndrome caused by the WRN gene mutation. It is characterized by a general appearance of premature aging, diabetes mellitus, and atherosclerosis, and an increased risk of malignancies. We report a patient who presented with
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