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Merck
CN
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文件

安全信息

W0138

Sigma-Aldrich

Western Blocker™溶液

for HRP detection systems

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别名:
Western blot blocking solution, blocking buffer
UNSPSC代码:
41105329
NACRES:
NA.25

质量水平

200

保质期

≥2 yr

储存温度

2-8°C

相关类别

一般描述

在蛋白质通过斑点印迹或蛋白质印迹转移到 PVDF 和硝酸纤维素膜上后，蛋白质印迹封闭液可用于封闭 PVDF 和硝酸纤维素膜。进行封闭是为了减少或消除一抗或二抗的非特异性结合。

应用

蛋白质印迹封闭液已针对使用辣根过氧化物酶（HRP）偶联物的检测系统进行了优化；它也与基于碱性磷酸酶的系统兼容。
蛋白质印迹封闭液已用于封闭蛋白质印迹膜。它也已用于蛋白质印迹中抗体的稀释。

特点和优势

  • 像许多基于牛奶的封闭液一样，它不会抑制弱 HRP 信号。
  • 既可以用作封闭成分，也可以用作探测成分。
  • 提供即用型，无需制备步骤。

法律信息

Western Blocker is a trademark of Sigma-Aldrich Co. LLC

法规信息

含少量动物源组分生物产品

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