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TRUE5

Sigma-Aldrich

TrueGel3D 水凝胶试剂盒

FAST-PVA, PEG non cell-degradable crosslinker

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.71

一般描述

The gel is formed by stable thioether bonds between maleimide groups of PVA (FAST-PVA) and thiol groups on PEG non cell-degradable crosslinker. Malemide-functionalized PVA is specifically used to decrease the time for gel formation. The PVA polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.

应用

TRUE5-1KT is preferred over dextran-based gels for long-term culture of cells which producing carbohydrate degrading enzymes. It is used to culture spheroids which proliferate but do not attach to hydrogel. PVA-PEG hydrogel kits are also used to immobilize cells or specimens for imaging.

特点和优势

  • Fast gelling (takes few seconds to minutes after addition of crosslinker)
  • Flexibility in modulating gel stiffness
  • Does not allow cell migration
  • Cell inert hydrogel (if not customized with RGD peptide)
  • Cells cannot be recovered

组分

TRUE5-1KT contains FAST-PVA (polymer) in solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol and water.

注意

Avoid prolong exposure of PEG non cell-degradable crosslinker to air and store Mal-PVA in small portions to avoid frequent freeze-thaw cycles.

象形图

Corrosion
GHS05

警示用语：

Warning

危险声明

H290

预防措施声明

P234 - P390

危险分类

Met. Corr. 1

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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