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安全信息

TRUE4

Sigma-Aldrich

TrueGel3D 水凝胶试剂盒

FAST-PVA, CD cell-degradable crosslinker

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.71

一般描述

The gel is formed by stable thioether bonds between maleimide groups of PVA (FAST-PVA) and thiol groups on CD cell-degradable crosslinker. Malemide-functionalized PVA is specifically used to decrease the time for gel formation. The PVA polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

应用

TRUE4-1KT is used for cell migration studies of amoeboid cells including neutrophils, dendritic cells and lymphocytes. It has been preferred to use PVA-based gels over dextran-based gels for long-term culture of cells producing carbohydrate degrading enzymes.

特点和优势

  • Fast gelling (takes few seconds to minutes after addition of crosslinker)
  • Flexibility in modulating gel stiffness
  • Contains MMP-cleavable crosslinker
  • Cells cannot be recovered
  • Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide)

组分

TRUE4-1KT contains FAST-PVA polymer solution in phosphate buffer and lyophilized CD cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol and water.

注意

Avoid prolong exposure of CD cell-degradable crosslinker to air and store FAST-PVA in small portions to avoid frequent freeze-thaw cycles.

象形图

Corrosion
GHS05

警示用语：

Warning

危险声明

H290

预防措施声明

P234 - P390

危险分类

Met. Corr. 1

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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商品

Utilization of TrueGel3D™ Hydrogels to Study Fibroblast Spreading

Fibroblasts that are present in the stroma secrete glycoproteins and polysaccharides to form extracellular matrix. They maintain the structural integrity within the connective tissue and play a key role in cancer, chronic obstructive pulmonary diseases, idiopathic pulmonary fibrosis, scleroderma and several other diseases.

True Gel3D™ Hydrogel Troubleshooting

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

3D细胞培养水凝胶_细胞培养_TrueGel3D™水凝胶-默克生命科学

TrueGel3D™细胞培养水凝胶是一种由混合聚合物与交联剂混合而成的生化成分限定的水凝胶。与其他基于动物细胞生物提取物（例如小鼠EHS肿瘤细胞）的水凝胶相比，TrueGel3D™细胞培养水凝胶不含任何可能会干扰或造成实验污染的动物源成分。

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

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实验方案

TrueGel3D™ Hydrogel Prep Protocol

TrueGel3D™ hydrogel enables cell encapsulation for 3D culture, mimicking native tissue environment with chemically defined properties.

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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