用途
sufficient for ~200 assays
质量水平
一般描述
This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.
应用
Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.
特点和优势
- Complete kit for protein assays
- Most widely cited protein assay
- Less protein to protein variation than other assays
- Contains reagents that can remove interfering substances
原理
An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3
靶器官
Respiratory system
储存分类代码
8A - Combustible corrosive hazardous materials
WGK
WGK 3
法规信息
新产品
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实验方案
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
相关内容
蛋白定量方法、试剂和免疫测定技术可准确测定各类样品中的蛋白浓度。
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
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