描述
wavelength 500-750nm
质量水平
用途
sufficient for ~50 manual assays
蛋白检测测量范围
0-1 mg/mL
参数
18-26 °C temperature
200 μL sample volume
储存温度
2-8°C
一般描述
双缩脲和 Lowry 程序用于蛋白质测定。前者广泛用于临床检测。后者尽管更灵敏，却只用于研究工作，受限于组合试剂的低稳定性、颜色的不可再现性（特别是在低蛋白浓度下）以及和蛋白质浓度的非线性显色反应。Ohnishi 和 Barr 改良了用于 Lowry 测定法的双缩脲试剂，从而简化了流程，同时提高了组合试剂的稳定性。
应用
全蛋白试剂盒（ Micro-Lowry, Onishi & Barr Modification ）已用于测定以下物质的总可溶蛋白质浓度:
- 麦醇溶蛋白的透析液/提取物
- 面粉（燕麦 和 硬粒小麦）和种子（藜麦, 芡欧鼠尾草）的提取物
- 储存的大鼠肝微粒体部分样品
原理
比色法，终点。 化验。Ohnishi 和 Barr′s 对微 Lowry 方法的改进。根据实验步骤，稀释的缩二脲试剂与肽键反应生成紫蓝色复合物，通过加入苯酚试剂增强其颜色。在 550-750 nm 处读取的吸光度用于确定标准曲线的结果。
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- B3934Biuret reagent, protein detection level 150-1,000 μg/mL 110 mL化学品安全说明书
- Folin & Ciocalteu’s phenol reagent 5 mL
自备
产品编号
说明
价格
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
易制毒化学品（2类）
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