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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
DM1A, monoclonal
分子量
antigen ~50 kDa
包含
15 mM sodium azide
种属反应性
yeast, mouse, amphibian, human, rat, chicken, fungi, bovine
增强验证
independent ( antibodies)
Learn more about Antibody Enhanced Validation
技术
indirect immunofluorescence: 1:500 using cultured chicken fibroblasts
western blot: 1:500 using human or chicken fibroblasts
同位素/亚型
IgG1
UniProt登记号
应用
research pathology
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)
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一般描述
抗α-微管蛋白(鼠IgG1亚型)单克隆抗体由DM1A 杂交瘤产生,这一杂交瘤由小鼠骨髓瘤细胞和免疫BALB/c小鼠的脾细胞融合产生。纯化的鸡脑微管可用作免疫原。通过使用小鼠单克隆抗体同种型试剂(产品号ISO2)和双扩散免疫检测,以确定同种型。产物是蛋白A纯化的与异硫氰酸荧光素缀合的单克隆抗-α-微管蛋白抗体异构体I。由凝胶过滤纯化,不含可检测出的游离FITC。小鼠单克隆抗-α-微管蛋白FITC抗体可特异性识别 α-微管蛋白羧基末端部分的表位。它可对人、猴、牛、鸡、山羊、鼠、大鼠、沙鼠、仓鼠、大鼠袋鼠、两栖动物、海胆、锥虫、酵母、真菌和烟草中的 α-微管蛋白进行定位。
特异性
免疫原
应用
小鼠源抗α-微管蛋白单克隆抗体可用于检测以下物质是否存在α-微管蛋白:
- 人骨肉瘤和乳腺癌细胞系(蛋白质印迹法)
- HeLa 细胞(通过免疫荧光显微镜观察)
- (免疫染色法)
- 非洲爪蟾(Xenopus )胚胎(免疫组织化学检测)
生化/生理作用
α/β-微管蛋白是微管的主要成分。这些细胞内、空心、圆柱形、丝状的结构几乎存在于所有真核细胞中。α/β-微管蛋白的自组装可导致极性微管结构由线性排列的交叉串状α- 和β-微管蛋白对指向了相同的方向构建而成。微管在有丝分裂、细胞内转运、睫状鞭毛运动以及细胞形状的产生和维持中起到了结构性和活动元素的作用。α/β-微管蛋白和γ-微管蛋白是蛋白微管蛋白超家族成员。α/β-微管蛋白是异二聚体,由一条 α-微管蛋白链和一条 β-微管蛋白链组成;每个亚基的分子量均为55 kDa,具有相当大的同源性。
储存及稳定性
如果更长时间储存,可将溶液按工作体积分装后冻存。不建议反复冻融或存放在“无霜”冰柜中。如果在更长时间的储存后出现了轻微的浑浊,在使用前可通过离心而使溶液澄清。
其他说明
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
从最新的版本中选择一种:
分析证书(COA)
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the immunogen for Monoclonal Anti-α-Tubulin antibody, clone DM1A?
The immunogen for Monoclonal Anti-α-Tubulin antibody produced in mouse, clone DM1A is microtubules from chicken embryo brain. This antibody recognizes an epitope located at the C-terminal end of the α-tubulin isoform (amino acids 426-430) as noted in the following references:Breitling, F., and Little, M., J. Molec. Biol., 189, 367-370 (1986).Wolff, A., et al., Biol. Cell, 63, 319-326 (1988).Serrano, L., et al., Anal. Biochem., 159, 253-259 (1986).
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商品
免疫荧光法(IF)通过在抗体标记上荧光分子,然后使用激光器激发荧光,包括蛋白质的定位、验证翻译后修饰或活化以及与其他蛋白质的邻近和复合等操作。
Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.
Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.
包含β-肌动蛋白、α-微管蛋白和波形蛋白用于细胞骨架结果蛋白活细胞分析的高滴度慢病毒颗粒。
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