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无菌性
sterile-filtered
质量水平
表单
solution
浓度
29.5 g/L in deionized water
技术
cell culture | mammalian: suitable
运输
ambient
储存温度
room temp
应用
除了用于需要复杂营养的微生物的生长外，我们还研究了磷锥虫肉汤（TPB）作为培养基制备的补充，该培养基支持BHK-21细胞中疫苗的生产和SF21昆虫细胞在高密度灌注培养搅拌罐生物反应器中的生长。
组分
胰蛋白酶磷酸盐肉汤（TPB）由四种成分组成：胰蛋白酶（20g/L）；葡萄糖（2g/L）；氯化钠（5g/L）和磷酸二钠（2.5g/L），通常调节至pH 7.3。胰蛋白酶组分是一种蛋白胨（氨基酸和短肽的混合物），由牛奶蛋白酪蛋白的混合酶水解（胰酶）得到。这种水解产物提供基于氨基酸的营养物和生存因子的来源，支持难养微生物如布鲁氏菌、链球菌和奈瑟氏球菌以及真核细胞如昆虫和动物细胞的生长。葡萄糖提供了一种可发酵的碳水化合物，可被需要复杂营养的微生物利用。氯化钠维持渗透和离子平衡，磷酸二钠提供基本缓冲能力。
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Héctor Puente et al.
Frontiers in microbiology, 11, 1911-1911 (2020-09-26)
Coronaviruses (CoVs) cause severe respiratory, enteric, and systemic infections in a wide range of hosts, including humans and animals. Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family, is the etiological agent of porcine epidemic diarrhea (PED), a
Dong-Hwan Kim et al.
Molecular biotechnology, 63(2), 140-149 (2021-01-03)
Selection of guide RNA (gRNA) is important to increase the efficiency of gene editing in the CRISPR/Cas9 system. Due to the variation in actual efficiency of insertion/deletion (indel) mutation among selected gRNAs in silico, reliable methods for validation of efficiency
S M Deutschmann et al.
Enzyme and microbial technology, 16(6), 506-512 (1994-06-01)
Spodoptera frugiperda insect cells (IPLB-Sf21-AE) (Sf21), infected with baculovirus expression vectors during their exponential growth phase, are commonly used to produce a variety of heterologous recombinant proteins. In the present study the culture conditions of these insect cells were studied
S N Saha et al.
Vaccine, 7(4), 357-363 (1989-08-01)
Studies were undertaken to develop a cheaper medium with indigenous sources of peptone and casein hydrolysate for continuous culture of BHK-21 (suspension) cells and production of foot-and-mouth disease (FMD) vaccine. Eleven batches of experimental media were prepared using different indigenous
A I Josemans et al.
Annals of the New York Academy of Sciences, 969, 141-146 (2002-10-17)
The in vitro culture of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants, was first achieved in 1985. Culture media were usually supplemented with serum and tryptose phosphate broth, both undefined components, contributing to great variability. Recently, we
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