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应用
除了用于需要复杂营养的微生物的生长外，我们还研究了磷锥虫肉汤（TPB）作为培养基制备的补充，该培养基支持BHK-21细胞中疫苗的生产和SF21昆虫细胞在高密度灌注培养搅拌罐生物反应器中的生长。
组分
胰蛋白酶磷酸盐肉汤（TPB）由四种成分组成：胰蛋白酶（20g/L）；葡萄糖（2g/L）；氯化钠（5g/L）和磷酸二钠（2.5g/L），通常调节至pH 7.3。胰蛋白酶组分是一种蛋白胨（氨基酸和短肽的混合物），由牛奶蛋白酪蛋白的混合酶水解（胰酶）得到。这种水解产物提供基于氨基酸的营养物和生存因子的来源，支持难养微生物如布鲁氏菌、链球菌和奈瑟氏球菌以及真核细胞如昆虫和动物细胞的生长。葡萄糖提供了一种可发酵的碳水化合物，可被需要复杂营养的微生物利用。氯化钠维持渗透和离子平衡，磷酸二钠提供基本缓冲能力。
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Dong-Hwan Kim et al.
Molecular biotechnology, 63(2), 140-149 (2021-01-03)
Selection of guide RNA (gRNA) is important to increase the efficiency of gene editing in the CRISPR/Cas9 system. Due to the variation in actual efficiency of insertion/deletion (indel) mutation among selected gRNAs in silico, reliable methods for validation of efficiency
Héctor Puente et al.
Frontiers in microbiology, 11, 1911-1911 (2020-09-26)
Coronaviruses (CoVs) cause severe respiratory, enteric, and systemic infections in a wide range of hosts, including humans and animals. Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family, is the etiological agent of porcine epidemic diarrhea (PED), a
S M Deutschmann et al.
Enzyme and microbial technology, 16(6), 506-512 (1994-06-01)
Spodoptera frugiperda insect cells (IPLB-Sf21-AE) (Sf21), infected with baculovirus expression vectors during their exponential growth phase, are commonly used to produce a variety of heterologous recombinant proteins. In the present study the culture conditions of these insect cells were studied
Faizal Z Asumda et al.
Differentiation; research in biological diversity, 101, 16-24 (2018-04-08)
A variety of approaches have been developed for the derivation of hepatocyte-like cells from pluripotent stem cells. Currently, most of these strategies employ step-wise differentiation approaches with recombinant growth-factors or small-molecule analogs to recapitulate developmental signaling pathways. Here, we tested
Mark Smyth et al.
Frontiers in immunology, 12, 638485-638485 (2021-07-02)
Cytotoxic T lymphocytes (CTLs) represent key immune effectors of the host response against chronic viruses, due to their cytotoxic response to virus-infected cells. In response to this selection pressure, viruses may accumulate escape mutations that evade CTL-mediated control. To study
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