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Merck
CN

T7791

胸腺嘧啶核苷5′-三磷酸 钠盐 溶液

10 mM (pH 7.0)

别名:

TTP

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化学文摘社编号:
18423-43-3
NACRES:
NA.52
PubChem Substance ID:
24900477
UNSPSC Code:
41106305
MDL number:
MFCD00080338

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产品名称

胸腺嘧啶核苷5′-三磷酸 钠盐 溶液, 10 mM (pH 7.0)

InChI

1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;

SMILES string

[Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O

InChI key

KAELIXTVSANDSE-UHFFFAOYSA-N

assay

≥99%

form

liquid

concentration

10 mM (pH 7.0)

color

colorless

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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相关类别

General description

胸苷5′-三磷酸钠盐溶液可用于DNA合成反应，如 PCR、DNA 测序、cDNA合成和分子克隆技术。

Application

Thymidine 5′-triphosphate sodium salt solution has been used:

  • as a standard to quantify each dNTP from cellular lysates by the liquid chromatography-mass spectrometry (LC-MS/MS) method
  • as a component of the dNTP mix for labeling of ribosomal 18s rDNA probe and telomeric (TTAGG)n probe
  • for routine PCR amplifications

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000474080
0000474080
SLCB6968
SLCB6968
SLBQ9779V

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Xinhui Chen et al.
Biomedical chromatography : BMC, 31(3) (2016-08-25)
The endogenous deoxynucleoside triphosphate (dNTP) pool includes deoxyadenosine triphosphate (dATP), deoxycytidine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP) and thymidine triphosphate (TTP). The endogenous dNTP pool is regulated by complex enzymatic pathways that can be targeted by drugs, such as antimetabolites. In
Michael G Mohsen et al.
Nucleic acids research, 47(18), 9495-9501 (2019-09-11)
We document the preparation and properties of dimerized pentaphosphate-bridged deoxynucleotides (dicaptides) that contain reactive components of two different nucleotides simultaneously. Importantly, dicaptides are found to be considerably more stable to hydrolysis than standard dNTPs. Steady-state kinetics studies show that the

实验方案

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

使用Jumpstart™Taq DNA聚合酶(D9307)扩增DNA

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.

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