推荐产品
生物来源
bovine pancreas
质量水平
类型
Type V-S
形式
powder
比活
≥8,500 BAEE units/mg protein (biuret)
UniProt登记号
储存温度
−20°C
基因信息
cow ... TRYP8(282603)
相关类别
一般描述
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
应用
Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.
生化/生理作用
Lys-acetylated trypsin is less susceptible to autolysis than the native form.
单位定义
One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).
制备说明
Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).
其他说明
View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.
底物
抑制剂
产品编号
说明
价格
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 3
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
动植物源性产品
常规特殊物品
Shahid Mehmood et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(27), 10832-10836 (2012-06-20)
The study of membrane proteins remains a challenging task, and approaches to unravel their dynamics are scarce. Here, we applied hydrogen/deuterium exchange (HDX) coupled to mass spectrometry to probe the motions of a bacterial multidrug ATP-binding cassette (ABC) transporter, BmrA
R F Regan et al.
Journal of neurochemistry, 70(1), 77-85 (1998-01-09)
The effect of magnesium on the oxidative neuronal injury induced by hemoglobin was assessed in murine cortical cell cultures. Exposure to 5 microM hemoglobin in physiologic (1 mM) magnesium for 26 h resulted in the death of about one-half the
Amy R Quinn et al.
The Journal of endocrinology, 214(3), 289-299 (2012-06-23)
Erratic regulation of glucose metabolism including hyperglycemia is a common condition in premature infants and is associated with increased morbidity and mortality. The objective of this study was to examine histological and ultrastructural differences in the endocrine pancreas in fetal
Shin-Ichi Nakakita et al.
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We describe a method to detect influenza virus using an evanescent-field-activated fluorescence scanner type glycan array and ELISA system. Neoglycoprotein was prepared by combination of organic chemistry and biomaterial preparation. These ligands were spotted on a glass plate or plastic
Nongluk Sriwilaijaroen et al.
Methods in molecular biology (Clifton, N.J.), 1200, 107-120 (2014-08-15)
The correlation between precise interactions of influenza A virus hemagglutinins with host cell surface glycans having terminal sialic acids and host range specificity has provoked the development of a high-throughput viral-receptor specificity assay. Here, we describe the use of the
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