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Merck
CN

B4500

Sigma-Aldrich

Nα-苯甲酰基-L-精氨酸乙酯 盐酸盐

trypsin substrate, chromogenic, ≥97% (HPLC), powder

别名:

BAEE

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About This Item

经验公式(希尔记法):
C15H22N4O3 · HCl
CAS号:
分子量:
342.82
Beilstein:
3781694
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.32

product name

Nα-苯甲酰基-L-精氨酸乙酯 盐酸盐, trypsin substrate

质量水平

检测方案

≥97% (HPLC)

形式

powder

溶解性

water: 50 mg/mL, clear, colorless

储存温度

2-8°C

SMILES字符串

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1

InChI key

HIXDELXKSSLIKB-YDALLXLXSA-N

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一般描述

Nα-苯甲酰基-L-精氨酸乙酯盐酸盐是胰蛋白酶底物。是用于光谱测定的非蛋白质底物。

应用

  • N

  • α-苯甲酰基-L-精氨酸乙酯盐酸盐作为底物,用于:
  • 在牙龈卟啉单胞菌( P. gingivalis )囊泡中测定肽基精氨酸脱亚氨酶(PPAD)
  • 测定番木瓜植物部位酶提取物的蛋白水解活性
  • 测定大鼠尾肌腱束的胰蛋白酶活性

底物

胰蛋白酶的原型底物。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

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在文件库中查找您最近购买产品的文档。

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Optima of Trypsin-Catalyzed Hydrolysis and Its Inhibition Determined by SDS-PAGE
Zhou X, et al.
Advances in Enzyme Research, 4(01), 1-1 (2016)
Kinetic and Structural Differentiation of Trypsin from Different Origins
Aithal M, et al.
BioPharm International, 30(1), 38-+-38-+ (2017)
Ying-Qing Yu et al.
Analytical chemistry, 75(21), 6023-6028 (2003-11-01)
Improved in-solution tryptic digestion of proteins in terms of speed and peptide coverage was achieved with the aid of a novel acid-labile anionic surfactant (ALS). Unlike SDS, ALS solubilizes proteins without inhibiting trypsin or other common endopeptidases activity. Trypsin activity
Chensong Pan et al.
Analytical and bioanalytical chemistry, 387(1), 193-204 (2006-11-07)
Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) is widely used in a variety of fields because it has the characteristics of speed, ease of use, high sensitivity, and wide detectable mass range for obtaining molecular weights and for structural characterization of
Mohammad K Hossain et al.
Colloids and surfaces. B, Biointerfaces, 181, 85-93 (2019-05-28)
Electric current responsive magnetic composite particles are prepared in three steps. In the first step, spherical and mesoporous submicrometer-sized magnetic iron oxide (Fe3O4) core particles are prepared by solvothermal method. Then magnetic Fe3O4 particles are functionalized with amine groups using

商品

木瓜蛋白酶是肽酶C1家族的一种半胱氨酸蛋白酶。它由单一多肽链组成,含有三个二硫键和一个酶活性所必需的巯基。

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

实验方案

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯(BAEE)作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定(A253,光程= 1cm)方案:

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

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