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生物来源
Serratia marcescens
质量水平
重组
expressed in E. coli
形式
liquid
浓度
≥200,000 units/mL
技术
DNA purification: suitable
适用性
suitable for cell lysis
应用
life science and biopharma
储存温度
−20°C
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相关类别
一般描述
来自粘质沙雷氏菌的内切酶是一种二聚体，包含两个具有不同蛋白质折叠的相同单体。核心包含一个反平行的六股β-片，两侧各有α-螺旋。每个单体具有一个活性位点。这种酶是一种依赖镁的核酸酶。
应用
来自粘质沙雷氏菌的碳化切酶已被用于邻近生物素化分析(BioID)和亲和纯化期间的细胞裂解。它也被用作裂解缓冲液的组分，以从细胞系中提取蛋白，用于亲和纯化研究。
碳化切酶在一项研究中用于评估TY3 gag3间隔物对细胞内冷凝和脱壳的影响。
用于去除蛋白样品中的核酸。
生化/生理作用
涡轮核酸切酶通过降低粘度和降解RNA、基因组DNA、杆状病毒DNA和未包壳载体DNA提供核酸酶处理。
粘质沙雷氏菌的核酸内切酶对单链和双链DNA和RNA均有效。它介导3个 O—P键的消化，产生以5个单磷酸结尾的寡核苷酸。已知这种酶的活性较少受还原剂和潮剂的影响。它在室温下高度稳定。这种内切酶在下游处理过程中可消除不需要的核酸。
消化天然或热变性的DNA和RNA。
单位定义
在pH 8.0，37 °C条件下，一个单位的酶可在30分钟内将超声鲑鱼精子DNA消化成酸溶性寡核苷酸，等同于ΔA260的1.0。
外形
以溶于50mM Tris-HCl (pH 8.0)和50 mM NaCl的溶液形式提供。
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
M D Miller et al.
Journal of molecular biology, 288(5), 975-987 (1999-05-18)
Serratia endonuclease is an important member of a class of magnesium dependent nucleases that are widely distributed in nature. Here, we describe the location and geometry of a magnesium-water cluster within the active site of this enzyme. The sole protein
Kristina Clemens et al.
Journal of virology, 85(7), 3055-3066 (2011-01-29)
Cells expressing the yeast retrotransposon Ty3 form concentrated foci of Ty3 proteins and RNA within which virus-like particle (VLP) assembly occurs. Gag3, the major structural protein of the Ty3 retrotransposon, is composed of capsid (CA), spacer (SP), and nucleocapsid (NC)
Madhuri Gade et al.
JACS Au, 1(12), 2349-2360 (2022-01-04)
Protein conformational changes can facilitate the binding of noncognate substrates and underlying promiscuous activities. However, the contribution of substrate conformational dynamics to this process is comparatively poorly understood. Here, we analyze human (hMAT2A) and Escherichia coli (eMAT) methionine adenosyltransferases that
Sylvain Cecchini et al.
Human gene therapy, 22(8), 1021-1030 (2011-03-09)
The large amounts of recombinant adeno-associated virus (rAAV) vector needed for clinical trials and eventual commercialization require robust, economical, reproducible, and scalable production processes compatible with current good manufacturing practice. rAAV produced using baculovirus and insect cells satisfies these conditions;
Aekkachai Tuekprakhon et al.
Cell, 185(14), 2422-2433 (2022-07-01)
The Omicron lineage of SARS-CoV-2, which was first described in November 2021, spread rapidly to become globally dominant and has split into a number of sublineages. BA.1 dominated the initial wave but has been replaced by BA.2 in many countries.
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