类型
Type I
质量水平
形式
ammonium sulfate suspension
比活
~10,000 units/mg protein
分子量
53 kDa by equilibrium centrifugation
异质活性
pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%
储存温度
2-8°C
相关类别
应用
已有研究使用磷酸丙糖异构酶来评估葡萄膜黑色素瘤的14种蛋白质的差异表达。 磷酸丙糖异构酶还被用于研究乙状结肠pH梯度在人内皮细胞蛋白质的自由流动等电聚焦中的应用。
单位定义
一个酶活性单位是指在pH 7.6、温度25 °C条件下，每分钟将1.0 μmole的D-甘油醛-3-磷酸转化为二羟丙酮磷酸所需的酶量。
外形
溶于2.7 M (NH4)2SO4溶液中的结晶悬浮液，含0.5 mM EDTA，pH 6.5
分析说明
通过缩二脲法测定的蛋白质。
其他说明
由于该酶的测定似乎对微小变量敏感，因此，按每mg蛋白质进行包装，而不是按单位包装。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Xiao Ma et al.
Electrophoresis, 33(9-10), 1349-1355 (2012-06-01)
Prefractionation of proteins enhances the resolution of proteome analysis of whole cells. Free-flow electrophoresis (FFE) provides a useful step in various prefractionation protocols, since matrix-free isoelectric focusing (FF-IEF) performed in this machine enables the enrichment of large, easily absorbable, sensitive
Annett Linge et al.
Investigative ophthalmology & visual science, 53(8), 4634-4643 (2012-05-10)
To compare the proteomic profiles of two categories of primary uveal melanoma tissue samples; those from patients who have subsequently developed metastatic disease and those who have not. Two-dimensional difference gel electrophoresis (2D DIGE) was performed on 25 uveal melanoma
Xiaochen He et al.
Journal of cellular physiology, 237(8), 3317-3327 (2022-05-28)
Hypertension is an important risk factor in the pathogenesis of diastolic dysfunction. Growing evidence indicates that glucose metabolism plays an essential role in diastolic dysfunction. TP53-induced glycolysis and apoptosis regulator (TIGAR) has been shown to regulate glucose metabolism and heart
Rongchang Yang et al.
Experimental parasitology, 137, 46-52 (2013-12-21)
A novel quantitative PCR (qPCR) for Giardia at the glutamate dehydrogenase (gdh) locus was developed and validated. The qPCR was used to screen a total of 3412 lamb faecal samples collected from approximately 1189 lambs at three sampling periods (weaning
Matthew J Nolan et al.
Water research, 47(5), 1726-1740 (2013-01-30)
There has been no large-scale systematic molecular epidemiological investigation of the waterborne protozoans, Cryptosporidium or Giardia, in southeastern Australia. Here, we explored, for the first time, the genetic composition of these genera in faecal samples from animals in nine Melbourne
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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