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T2507

磷酸丙糖异构酶 来源于面包酵母（酿酒酵母）

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

别名:

D-甘油醛-3-磷酸酮醇异构酶, TPI

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关于此项目

化学文摘社编号:
9023-78-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-774-2
MDL number:
MFCD00082080
EC 号:
5.3.1.1 (BRENDA, IUBMB)
Specific activity:
~10,000 units/mg protein
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type

Type I

Quality Level

200

form

ammonium sulfate suspension

specific activity

~10,000 units/mg protein

mol wt

53 kDa by equilibrium centrifugation

foreign activity

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%

storage temp.

2-8°C

Application

已有研究使用磷酸丙糖异构酶来评估葡萄膜黑色素瘤的14种蛋白质的差异表达。 磷酸丙糖异构酶还被用于研究乙状结肠pH梯度在人内皮细胞蛋白质的自由流动等电聚焦中的应用。

Physical form

溶于2.7 M (NH4)2SO4溶液中的结晶悬浮液，含0.5 mM EDTA，pH 6.5

Analysis Note

通过缩二脲法测定的蛋白质。

Other Notes

一个酶活性单位是指在pH 7.6、温度25 °C条件下，每分钟将1.0 μmole的D-甘油醛-3-磷酸转化为二羟丙酮磷酸所需的酶量。
由于该酶的测定似乎对微小变量敏感，因此，按每mg蛋白质进行包装，而不是按单位包装。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
036H8025
060H8110

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How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

如何使用溶于硫酸铵悬液的酶

以硫酸铵悬浮液形式提供的酶的使用指南

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