product name
Sigma 7-9®®, ≥99% (titration), crystalline
描述
aminopeptidase substrate
质量水平
检测方案
≥99% (titration)
形式
crystalline
储存条件
dry at room temperature
技术
ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
杂质
≤0.5% water (Karl Fischer)
颜色
white to off-white
pH值（酸碱度）
10.5-12
有效pH范围
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
溶解性
water: 0.667 g/mL, clear, colorless to faintly yellow
吸光度
≤0.4 at 290 at 40%
应用
clinical research
diagnostic assay manufacturing
life science and biopharma
sample preparation
SMILES字符串
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
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一般描述
Sigma 7-9® 也称为 Trizma 碱或三（羟甲基）氨基甲烷，是一种多功能生物缓冲液，可用于多种应用，例如蛋白质冷冻、电泳以及 TAE 和 TBE 等缓冲溶液的制备。Tris 的 pKa 为 8.06，对于在生物体典型生理 pH 范围（pH 7.0-9.0）内工作的实验室特别有价值。它还通常与其他缓冲剂联合用于精确调节溶液的 pH 值。
此外，Tris 有助于增强细胞膜的渗透性。这种化合物在其用于制备与生物体液相容的缓冲液，作为标准 pH 溶液，促进乳酸脱氢酶测定、原位杂交和细胞蛋白质提取等实验方案的实验室中不可或缺。
此外，Tris 有助于增强细胞膜的渗透性。这种化合物在其用于制备与生物体液相容的缓冲液，作为标准 pH 溶液，促进乳酸脱氢酶测定、原位杂交和细胞蛋白质提取等实验方案的实验室中不可或缺。
应用
Sigma 7-9® 已用于：
- ：作为样品制备的缓冲液，用于涉及酶联免疫吸附测定 (ELISA) 的研究
- 作为样品制备的缓冲液，用于免疫组织化学和免疫荧光研究
特点和优势
- 适用于生物和生化研究
- 在 pH 7-9 (25 °C)范围内有效缓冲，pKa 为 8.1 (25 °C)
其他说明
所有缓冲液的 pH 值都取决于温度和浓度。Tris 缓冲液，温度每降低 1°C，pH 值增加约 0.03 个单位，每稀释十倍 pH 值降低0.03-0.05 个单位。
对于精确的应用，使用经过精心校准的 pH 计和玻璃/甘汞组合电极。
对于精确的应用，使用经过精心校准的 pH 计和玻璃/甘汞组合电极。
法律信息
Sigma 7-9 is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
可比产品
产品编号
说明
价格
WGK
WGK 1
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
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