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T1378

Sigma-Aldrich

Sigma 7-9®®

≥99% (titration), crystalline, Sigma 7-9®

别名:

Trizma ® 碱, 2-氨基-2-(羟甲基)-1,3-丙二醇, THAM, Tris 碱, 三羟甲基氨基甲烷, 氨基丁三醇

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About This Item

线性分子式:
NH2C(CH2OH)3
CAS号:
77-86-1
分子量:
121.14
Beilstein:
741883
EC 号:
201-064-4
MDL编号:
MFCD00004679
UNSPSC代码:
12352104
PubChem化学物质编号:
24899961
NACRES:
NA.25

产品名称

Sigma 7-9®®, ≥99% (titration), crystalline

描述

aminopeptidase substrate

质量水平

200

方案

≥99% (titration)

表单

crystalline

储存条件

dry at room temperature

技术

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable

杂质

≤0.5% water (Karl Fischer)

颜色

white to off-white

pH值（酸碱度）

10.5-12

有效pH范围

7-9

pKa (25 °C)

8.1

沸点

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

溶解性

water: 0.667 g/mL, clear, colorless to faintly yellow

吸附

≤0.4 at 290 at 40%

应用

clinical research
diagnostic assay manufacturing
life science and biopharma
sample preparation

SMILES字符串

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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相关类别

一般描述

Sigma 7-9® 也称为 Trizma 碱或三（羟甲基）氨基甲烷，是一种多功能生物缓冲液，可用于多种应用，例如蛋白质冷冻、电泳以及 TAE 和 TBE 等缓冲溶液的制备。Tris 的 pKa 为 8.06，对于在生物体典型生理 pH 范围（pH 7.0-9.0）内工作的实验室特别有价值。它还通常与其他缓冲剂联合用于精确调节溶液的 pH 值。

此外，Tris 有助于增强细胞膜的渗透性。这种化合物在其用于制备与生物体液相容的缓冲液，作为标准 pH 溶液，促进乳酸脱氢酶测定、原位杂交和细胞蛋白质提取等实验方案的实验室中不可或缺。

应用

Sigma 7-9® 已用于：

  • ：作为样品制备的缓冲液，用于涉及酶联免疫吸附测定 (ELISA) 的研究
  • 作为样品制备的缓冲液，用于免疫组织化学和免疫荧光研究

特点和优势

  • 适用于生物和生化研究
  • 在 pH 7-9 (25 °C)范围内有效缓冲，pKa 为 8.1 (25 °C)

其他说明

所有缓冲液的 pH 值都取决于温度和浓度。Tris 缓冲液，温度每降低 1°C，pH 值增加约 0.03 个单位，每稀释十倍 pH 值降低0.03-0.05 个单位。
对于精确的应用，使用经过精心校准的 pH 计和玻璃/甘汞组合电极。
如需了解生化试剂系列的更多信息，请填写此表。

法律信息

Sigma 7-9 is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

可比产品

产品编号
说明
价格

T6687

缓血酸胺, meets USP testing specifications

T6791

Trizma ® 碱, BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)

T1503

Trizma ® 碱, Primary Standard and Buffer, ≥99.9% (titration), crystalline

T6066

Trizma ® 碱, BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

T4661

Trizma ® 碱, ≥99.9% (titration), crystalline

93350

Trizma ® 碱, puriss. p.a., ≥99.7% (T)

93352

Trizma ® 碱, ≥99.0% (T)

93362

Trizma ® 碱, BioUltra, for molecular biology, ≥99.8% (T)

33742

Trizma ® 碱, puriss. p.a., buffer substance, ≥99.5%

154563

三（羟甲基）氨基甲烷, ultrapure grade, ≥99.9%

252859

三（羟甲基）氨基甲烷, ACS reagent, ≥99.8%

T1699

Trizma ® 碱 溶液, 1.5 M

RDD008

Trizma ® 碱, anhydrous, free-flowing, Redi-Dri™, ≥99.9%

储存分类代码

11 - Combustible Solids

WGK

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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分析证书(COA)

Lot/Batch Number
0000412815
0000412815
0000412813
0000412813
0000407252

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