推荐产品
产品名称
三（羟甲基）氨基甲烷, ACS reagent, ≥99.8%
等级
ACS reagent
质量水平
方案
≥99.8%
99.8-100.1%
表单
crystalline
技术
affinity chromatography: suitable
杂质
≤0.005% insolubles
≤2% water
颜色
white
有效pH范围
7-9
pKa (25 °C)
8.1
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此商品 | 179191 | 589572 | C100307 |
|---|---|---|---|
| assay 99.5% | assay ≥99% | assay 99.5% | assay ≥99.8% |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| grade anhydrous | grade ACS reagent | grade anhydrous | grade Laboratory Reagent |
| form liquid | form liquid | form - | form liquid |
| autoignition temp. 500 °F | autoignition temp. 500 °F | autoignition temp. 500 °F | autoignition temp. 500 °F |
| impurities <0.001% water, <0.005% water (100 mL pkg) | impurities H2SO4, passes test (darkened), ≤0.02% water | impurities - | impurities - |
一般描述
三羟甲基氨基甲烷，常称为氨丁三醇、Tris 碱或Trizma碱，作为生物缓冲液在各种研究应用中发挥着至关重要的作用。由于8.1的最佳 pKa ，它被广泛用于Tris-乙酸盐-EDTA(TAE) 和Tris-硼酸盐-EDTA(TBE)等缓冲液配方，适用于将 pH 值维持在大多数生物体的生理范围内 (pH 7-9)。但是在研究一些蛋白质时务必谨慎，因为 Tris 碱可能会干扰某些酶的活性。Tris碱可单独用作缓冲液，也可用作混合缓冲液（包括Tris-EDTA (TE)缓冲液、TAE缓冲液和TBE缓冲液）配方的成分。重要的是，Tris碱的特征在于其纯度、稳定性和相对无吸湿性的性质。
在实验室环境中，Tris碱是制备与生物液相容的缓冲液必不可少的试剂，可作为标准pH 溶液。它有助于进行多种操作技术，如乳酸脱氢酶测试、原位杂交和细胞蛋白质提取。其多样化用途涵盖了细胞培养、生物化学和分子生物实验室应用，有助于细胞膜渗透性相关研究和缓冲液制备。
在实验室环境中，Tris碱是制备与生物液相容的缓冲液必不可少的试剂，可作为标准pH 溶液。它有助于进行多种操作技术，如乳酸脱氢酶测试、原位杂交和细胞蛋白质提取。其多样化用途涵盖了细胞培养、生物化学和分子生物实验室应用，有助于细胞膜渗透性相关研究和缓冲液制备。
应用
特点和优势
- 7-9 pH 范围内的高效缓冲能力，pKa为8.1 (25 °C)
- 经测试证实重金属污染浓度低，从而确保其适用于各种应用
- 可用于细胞生物学和生物化学研究
法律信息
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
可比产品
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
An optimized lactate dehydrogenase release assay for screening of drug candidates in neuroscience.
Kaja S et al.
Journal of Pharmacological and Toxicological Methods, 73, 1-1 (2015)
Tris-tris-HCl: a standard buffer for use in the physiologic pH range.
R A Durst et al.
Clinical chemistry, 18(3), 206-208 (1972-03-01)
Jianbin Zhang et al.
Autophagy, 10(5), 901-912 (2014-03-29)
Autophagy is a catabolic process during which cellular components including protein aggregates and organelles are degraded via a lysosome-dependent process to sustain metabolic homeostasis during nutrient or energy deprivation. Measuring the rate of proteolysis of long-lived proteins is a classical
A Reliable Protocol for In situ microRNAs Detection in Feeding Sites Induced by Root-Knot Nematodes.
Fernando E Díaz-Manzano et al.
Frontiers in plant science, 7, 966-966 (2016-07-28)
Galls induced by Meloidogyne spp. in plant roots are a complex organ formed by heterogeneous tissues; within them there are 5-8 giant cells (GCs) that root-knot nematodes use for their own nurturing. Subtle regulatory mechanisms likely mediate the massive gene
Sylvain Clède et al.
Chemical communications (Cambridge, England), 48(62), 7729-7731 (2012-06-29)
A rhenium tris-carbonyl derivative has been designed to couple infrared and luminescent detection in cells. Both spectroscopies are consistent with one another; they point out the reliability of the present SCoMPI (for Single Core Multimodal Probe for Imaging) for bimodal
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