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Merck
CN

STRN250

Sigma-Aldrich

Spectrum 植物总RNA试剂盒

greener alternative

sufficient for 250 purifications

别名:

Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit

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About This Item

UNSPSC代码:
41105501
NACRES:
NA.52

用途

sufficient for 250 purifications

质量水平

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技术

RNA purification: suitable

环保替代产品分类

储存温度

15-25°C

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一般描述

Spectrum植物总RNA试剂盒利用了裂解液、结合化学反应法以及方便的柱式“结合-洗涤-洗脱”形式,可在大约30分钟内从100 mg组织中纯化出多达100 μg总RNA。典型得率范围为20–60 μg。

在液氮中将组织研磨成细粉末之后,细胞裂解,细胞残渣通过物理和化学方法与内源性RNA分离。随后,RNA结合到柱式支撑硅胶底物上,经过几遍洗涤去除污染物。

从柱上洗脱总RNA,然后在Northern杂交、RT-PCR和qRT-PCR等常规应用中使用。
我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。本产品含有本安型化学制剂。

应用

Spectrum®植物总RNA试剂盒已用于:
  • 从液氮研磨材料中提取RNA
  • 从突变和野生植株中提取总RNA
  • 从叶片样品中提取总RNA
  • 从所有植物组织中提取RNA

特点和优势

  • 每次制备至多可获得60 μg高纯度浓缩RNA
  • 实验方案高度有效,可在30分钟内完成RNA纯化
  • 专为研究困难的植物组织而设计

法律信息

Spectrum is a trademark of Sigma-Aldrich Co. LLC

警示用语:

Danger

危险分类

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

靶器官

Liver,Heart

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

法规信息

危险化学品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Genome-wide transcriptomic analysis of the sporophyte of the moss Physcomitrella patens.
O'Donoghue MT
Journal of Experimental Botany, 64(12), 3567-3581 (2013)
Knockout mutants as a tool to identify the subunit composition of Arabidopsis glutamine synthetase isoforms
Dragicevic M, et al.
Plant Physiology and Biochemistry, 79(6), 1-9 (2014)
Jun Chen et al.
Genetics, 191(3), 865-881 (2012-05-01)
Understanding the genetic basis of local adaptation is challenging due to the subtle balance among conflicting evolutionary forces that are involved in its establishment and maintenance. One system with which to tease apart these difficulties is clines in adaptive characters.
Martin-Timothy O'Donoghue et al.
Journal of experimental botany, 64(12), 3567-3581 (2013-07-28)
Bryophytes, the most basal of the extant land plants, diverged at least 450 million years ago. A major feature of these plants is the biphasic alternation of generations between a dominant haploid gametophyte and a minor diploid sporophyte phase. These
Transcriptomic Analysis of Drought-Sensitive and Tolerant Genotypes of Perennial Ryegrass (Lolium perenne L.)
Breeding Grasses and Protein Crops in the Era of Genomics, 79(6), 160-165 (2018)

相关内容

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

DNA和RNA提取是分子生物学中的一种基本方法。高质量、高纯度的核酸在一系列研究和临床应用中发挥着重要的作用。因此,核酸纯化是分子生物学和基因组工作流程中的第一步。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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