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安全信息

STRN250

Sigma-Aldrich

Spectrum™ 植物总RNA试剂盒

greener alternative

sufficient for 250 purifications

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别名:
Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
UNSPSC代码:
12352200
NACRES:
NA.52

用途

sufficient for 250 purifications

质量水平

200

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技术

RNA purification: suitable

环保替代产品分类

Aligned,

储存温度

15-25°C

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相关类别

一般描述

Spectrum植物总RNA试剂盒利用了裂解液、结合化学反应法以及方便的柱式“结合-洗涤-洗脱”形式，可在大约30分钟内从100 mg组织中纯化出多达100 μg总RNA。典型得率范围为20–60 μg。

在液氮中将组织研磨成细粉末之后，细胞裂解，细胞残渣通过物理和化学方法与内源性RNA分离。随后，RNA结合到柱式支撑硅胶底物上，经过几遍洗涤去除污染物。

从柱上洗脱总RNA，然后在Northern杂交、RT-PCR和qRT-PCR等常规应用中使用。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。本产品含有本安型化学制剂。

应用

Spectrum®植物总RNA试剂盒已用于：
  • 从液氮研磨材料中提取RNA
  • 从突变和野生植株中提取总RNA
  • 从叶片样品中提取总RNA
  • 从所有植物组织中提取RNA

特点和优势

  • 每次制备至多可获得60 μg高纯度浓缩RNA
  • 实验方案高度有效，可在30分钟内完成RNA纯化
  • 专为研究困难的植物组织而设计

法律信息

Specac is a registered trademark of Specac Ltd.
Spectrum is a trademark of Sigma-Aldrich Co. LLC

警示用语：

Danger

危险分类

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

靶器官

Liver,Heart

WGK

WGK 3

法规信息

危险化学品

分析证书(COA)

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Martin-Timothy O'Donoghue et al.
Journal of experimental botany, 64(12), 3567-3581 (2013-07-28)
Bryophytes, the most basal of the extant land plants, diverged at least 450 million years ago. A major feature of these plants is the biphasic alternation of generations between a dominant haploid gametophyte and a minor diploid sporophyte phase. These
Anantha Peramuna et al.
Biochemical and biophysical research communications, 500(2), 418-422 (2018-04-17)
Securing a molecular toolbox including diverse promoters is essential for genome engineering. However, native promoters have limitations such as the available number or the length of the promoter. In this work, three short synthetic promoters were characterized by using the
Zhonghua Li et al.
The New phytologist, 226(6), 1738-1752 (2020-02-06)
The cotton fibre serves as a valuable experimental system to study cell wall synthesis in plants, but our understanding of the genetic regulation of this process during fibre development remains limited. We performed a genome-wide association study (GWAS) and identified
Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness
Minina EA, et al.
Journal of Experimental Botany, 69(6), 1415-1432 (2018)
Gregory S Jensen et al.
Journal of experimental botany, 68(3), 499-511 (2017-02-17)
Thigmomorphogenesis is a stereotypical developmental alteration in the plant body plan that can be induced by repeatedly touching plant organs. To unravel how plants sense and record multiple touch stimuli we performed a novel forward genetic screen based on the
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Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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