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STRN10

Sigma-Aldrich

Spectrum™ 植物总RNA试剂盒

greener alternative

sufficient for 10 purifications

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别名:
Plant Total RNA Kit
UNSPSC代码:
12352200
NACRES:
NA.52

用途

sufficient for 10 purifications

质量水平

200

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技术

RNA purification: suitable

环保替代产品分类

, Aligned

储存温度

15-25°C

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相关类别

一般描述

Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 （The 12 Principles of Greener Chemistry），更环保的替代产品。本产品符合固有安全化学原则(Inherently Safer Chemistry)
Spectrum植物总RNA试剂盒利用了裂解液、结合化学反应法以及方便的柱式“结合-洗涤-洗脱”形式，可在大约30分钟内从100 mg组织中纯化出多达100 μg总RNA。典型得率范围为20–60 μg。

在液氮中将组织研磨成细粉末之后，细胞裂解，细胞残渣通过物理和化学方法与内源性RNA分离。随后，RNA结合到柱式支撑硅胶底物上，经过几遍洗涤去除污染物。

从柱上洗脱总RNA，然后在Northern杂交、RT-PCR和qRT-PCR等常规应用中使用。

特点和优势

  • 每次制备至多可获得60 μg高纯度浓缩RNA
  • 实验方案高度有效，可在30分钟内完成RNA纯化
  • 专为研究困难的植物组织而设计

法律信息

Spectrum is a trademark of Sigma-Aldrich Co. LLC

警示用语：

Danger

危险分类

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

靶器官

Liver,Heart

WGK

WGK 3

闪点(°F)

154.4 °F

闪点(°C)

68 °C

法规信息

危险化学品

分析证书(COA)

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Elsa Góngora-Castillo et al.
Methods in enzymology, 517, 139-159 (2012-10-23)
Development of next-generation sequencing, coupled with the advancement of computational methods, has allowed researchers to access the transcriptomes of recalcitrant genomes such as those of medicinal plant species. Through the sequencing of even a few cDNA libraries, a broad representation
Neeraja Cherukupalli et al.
Frontiers in plant science, 7, 1203-1203 (2016-09-02)
Andrographis paniculata is an important medicinal plant containing various bioactive terpenoids and flavonoids. Despite its importance in herbal medicine, no ready-to-use transcript sequence information of this plant is made available in the public data base, this study mainly deals with
Zhandong Cai et al.
Frontiers in plant science, 11, 724-724 (2020-06-26)
Cadmium (Cd) is a widespread pollutant that is toxic to living organisms. Previous studies have identified certain WRKY transcription factors, which confer Cd tolerance in different plant species. In the present study, we have identified 29 Cd-responsive WRKY genes in
Qun Cheng et al.
Frontiers in plant science, 11, 272-272 (2020-04-08)
Soybean [Glycine max (L.) Merr.] is an important crop for oil and protein resources worldwide, and its farming is impacted by increasing soil salinity levels. In Arabidopsis the gene EARLY FLOWERING 3 (ELF3), increased salt tolerance by suppressing salt stress
Genomic approaches for interrogating the biochemistry of medicinal plant species.
Gongora-Castillo E
Methods in Enzymology, 517, 139-159 (2012)
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相关内容

Spectrum™ Plant Total RNA Kit

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

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