生物来源
mouse
重组
expressed in E. coli
标签
His tagged
检测方案
>95% (SDS-PAGE)
形式
liquid
分子量
~24 kDa
包装
pkg of 20 μg
浓度
≥0.2 mg/mL
杂质
<0.01 EU/μg endotoxin, tested
颜色
clear
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
mouse ... Tnfsf10(22035)
一般描述
Tumor Necrosis Factor (TNF)-Related Apoptosis-Inducing Ligand (TRAIL) is a type II transmembrane protein and belongs to the TNF superfamily of ligands. Trimerisation of TRAIL around a centrally positioned Zn atom is needed for maintaining its stability, solubility and biological activity as a death ligand. TRAIL exists in two forms, soluble and membrane bound.
生化/生理作用
Tumor Necrosis Factor (TNF)-Related Apoptosis-Inducing Ligand (TRAIL) activates the extrinsic apoptotic pathway by binding death receptors (DR) 4 and 5. In addition to DR 4 and 5, TRAIL activates several other receptors such as decoy receptor (DcR)1, DcR2 and a soluble receptor called osteoprotegerin. TRAIL plays a vital role in the inhibition of autoimmune inflammation and cell cycle progression. TRAIL selectively kills tumor cells without damaging normal tissues and therefore, it might act as a tumor-selective anticancer agent. TRAIL-induced apoptosis is involved in various cellular processes including activation-induced cell death, homeostasis, intrathymic negative selection, autoimmunity suppression, and immune surveillance. Repeated dosage of recombinant soluble TRAIL triggers apoptosis, inhibits tumor progression, and increases viability of mice suffering from cancer.
外形
Solution in 20 mM HEPES, pH 7.4, 300 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT.
其他说明
The extracellular domain of mouse TRAIL (aa 99-291) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is an inhibitor of autoimmune inflammation and cell cycle progression.
The Journal of Experimental Medicine, 191(7), 1095-1104 (2000)
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induced mitochondrial pathway to apoptosis and caspase activation is potentiated by phospholipid scramblase-3.
Apoptosis, 13(7), 845-856 (2008)
Overexpression of soluble TRAIL induces apoptosis in human lung adenocarcinoma and inhibits growth of tumor xenografts in nude mice.
Cancer Research, 65(5), 1687-1692 (2005)
Exosomes secreted by human placenta carry functional Fas ligand and TRAIL molecules and convey apoptosis in activated immune cells, suggesting exosome-mediated immune privilege of the fetus.
Journal of Immunology, 191(11), 5515-5523 (2013)
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门